Universidade Estadual de Londrina

About: Universidade Estadual de Londrina is a education organization based out in Londrina, Brazil. It is known for research contribution in the topics: Population & Context (language use). The organization has 13052 authors who have published 19291 publications receiving 212123 citations.

Genetic characterization of Brazilian bovine viral diarrhea virus isolates by partial nucleotide sequencing of the 5'-UTR region

Abstract: Nineteen isolates of bovine viral diarrhea virus (BVDV) from Brazil were genetically characterized through partial nucleotide sequencing and analysis of the 5'UTR region. The isolates were grouped as BVDV-1 (11/19), BVDV-2 (6/19) or "atypical" pestivirus (2/19). Among the BVDV-1, eight isolates were classified as subgenotype BVDV-1a, whereas most (4 out of 6) BVDV-2 belonged to subgenotype 2b. Two isolates from aborted fetuses were not classified into any genetic group, being considered atypical BVDVs. Genetic diversity among Brazilian BVDV isolates may be responsible for vaccination and diag-nostic failure and therefore may influence the control strategies for BVDV infection in the country.

Bovine papillomavirus type 13 DNA in equine sarcoids.

Abstract: Equine sarcoids are locally aggressive fibroblastic neoplasms considered to be the most common skin tumors of horses worldwide. Bovine papillomavirus types 1 and 2 have typically been associated with sarcoids in equids. Investigations aiming to identify papillomavirus strains, aside from bovine papillomaviruses 1 and 2, which might be associated with sarcoid lesions, have been lacking. The aim of this article is to report the identification of a third bovine papillomavirus type, bovine papillomavirus 13, associated with equine sarcoids. Six sarcoid lesions were collected from diverse anatomical sites on two horses from southern Brazil. To detect a broad spectrum of papillomavirus strains, eight degenerate primer pairs designed to detect conserved regions on the L1 and E1 genes were tested on the DNA samples. Direct sequencing was then performed on the obtained amplicons, and sequence identities were compared with sequences from all bovine papillomavirus types. The FAP59/FAP64, MY09/MY11, and AR-E1F2/AR-E1R4 sequences generated from the sarcoids were shown to present 99 to 100% identity with bovine papillomavirus 13, a new bovine papillomavirus type previously described in cattle. The results from this study suggest that there is a need to identify bovine papillomavirus type 13 and other papillomavirus strains that might be associated with sarcoids in diverse geographical areas; such investigations might establish the frequency of occurrence of this viral type in these common tumors of equids.

Extension of the core map of common bean with EST-SSR, RGA, AFLP, and putative functional markers

Abstract: Microsatellites and gene-derived markers are still underrepresented in the core molecular linkage map of common bean compared to other types of markers. In order to increase the density of the core map, a set of new markers were developed and mapped onto the RIL population derived from the ‘BAT93’ × ‘Jalo EEP558’ cross. The EST-SSR markers were first characterized using a set of 24 bean inbred lines. On average, the polymorphism information content was 0.40 and the mean number of alleles per locus was 2.7. In addition, AFLP and RGA markers based on the NBS-profiling method were developed and a subset of the mapped RGA was sequenced. With the integration of 282 new markers into the common bean core map, we were able to place markers with putative known function in some existing gaps including regions with QTL for resistance to anthracnose and rust. The distribution of the markers over 11 linkage groups is discussed and a newer version of the common bean core linkage map is proposed.

Adaptations on jump capacity in Brazilian volleyball players prior to the under-19 World Championship.

Abstract: The under-19 Brazilian volleyball national team has achieved great performances at international competitions. Because the vertical jump capacity is critical for success in volleyball, the purpose of this study was to identify the training-induced adaptations on jump capacity assessed by general and specific tests during 3 different moments (i.e., T1, T2, and T3) of a macrocycle of preparation for the world championship. The sample was composed of 11 athletes from the Brazilian national team-World Champion (age, 18.0 +/- 0.5 years; height: 198.7 +/- 5.4 cm; and body mass, 87.3 +/- 5.9 kg). They were evaluated for jumping capacity by the following tests: squat jump (SJ), countermovement jump (CMJ), and jump anaerobic resistance (15 seconds) (JAR) and standing reach, height, and vertical jump tests for attack and block. Descriptive statistics were computed, and a repeated-measures analysis of variance was used. The Tukey-Kramer post hoc test was used when appropriate. Significance was set at P < or = 0.05. The results showed that the training-induced adaptations on the SJ (3.9%) and CMJ (2.3%) were not statistically significant. The JAR showed statistical significance between T2 and T3 (9.6%), while the attack height and block height presented significant differences between T1 and T2 (2.5% and 3.3%, respectively) and T1 and T3 (3.0% and 3.5%, respectively). The volume of training was quantified between weeks 1 and 9 (10,750 minutes, 1,194 +/- 322 min x wk(-1)) and between weeks 10 and 18 (8,722 minutes, 969 +/- 329 min x wk(-1)). In conclusion, this study showed that there were progressive and significant training-induced adaptations, mainly on the tests that simulated the specific skills, such as spike and block, with the best results being reached after the first 9 weeks of training. This probably reflected not only the individual's capacity to adapt, but also the characteristics of the training loads prescribed during the entire macrocycle.