Journal ArticleDOI
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Jens Boch,Heidi Scholze,Sebastian Schornack,Angelika Landgraf,Simone Hahn,Sabine Kay,Thomas Lahaye,Anja Nickstadt,Ulla Bonas +8 more
TLDR
The functionality of a distinct type of DNA binding domain is described and allows the design ofDNA binding domains for biotechnology.Abstract:
The pathogenicity of many bacteria depends on the injection of effector proteins via type III secretion into eukaryotic cells in order to manipulate cellular processes. TAL (transcription activator-like) effectors from plant pathogenic Xanthomonas are important virulence factors that act as transcriptional activators in the plant cell nucleus, where they directly bind to DNA via a central domain of tandem repeats. Here, we show how target DNA specificity of TAL effectors is encoded. Two hypervariable amino acid residues in each repeat recognize one base pair in the target DNA. Recognition sequences of TAL effectors were predicted and experimentally confirmed. The modular protein architecture enabled the construction of artificial effectors with new specificities. Our study describes the functionality of a distinct type of DNA binding domain and allows the design of DNA binding domains for biotechnology.read more
Citations
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Journal ArticleDOI
Targeted promoter editing for rice resistance to Xanthomonas oryzae pv. oryzae reveals differential activities for SWEET14‐inducing TAL effectors
Servane Blanvillain-Baufumé,Maik Reschke,Montserrat Solé,Florence Auguy,Hinda Doucoure,Boris Szurek,Donaldo Meynard,Murielle Portefaix,Sébastien Cunnac,Emmanuel Guiderdoni,Jens Boch,Ralf Koebnik +11 more
TL;DR: This work offers the first set of alleles edited in TalC EBE and uncovers a distinct, broader range of activities for TalC compared to AvrXa7 or Tal5, suggesting the existence of additional targets forTalC beyond SWEET14, suggesting that TALE‐mediated plant susceptibility may result from induction of several, genetically redundant, host susceptibility genes by a single effector.
Journal ArticleDOI
Highly efficient and specific genome editing in silkworm using custom TALENs.
Sanyuan Ma,Shengling Zhang,Feng Wang,Yong Liu,Yuanyuan Liu,Hanfu Xu,Chun Liu,Ying Lin,Ping Zhao,Qingyou Xia +9 more
TL;DR: It is demonstrated that (i) TALENs can be used in silkworm and (ii) heritable large chromosomal deletions can be induced by two pairs of TALens in whole organisms.
Journal ArticleDOI
Synthetic gene expression perturbation systems with rapid, tunable, single-gene specificity in yeast
TL;DR: Two artificial transcription factors (ATFs) are engineered that contain Cys2His2 zinc-finger DNA-binding domains of either the mouse transcription factor Zif268 or a rationally designed array of four zinc fingers and can rapidly induce a single gene driven by a synthetic promoter in response to introduction of an otherwise inert hormone with no detectable off-target effects.
Patent
Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
Feng Zhang,Chie-yu Lin,Fei Ran +2 more
TL;DR: In this article, the authors provide delivery, engineering and optimization of systems, methods, and compositions for manipulation of sequences and/or activities of target sequences, including vectors and vector systems, some of which encode one or more components of a CRISPR complex.
Journal ArticleDOI
Programmable RNA editing by recruiting endogenous ADAR using engineered RNAs.
Liang Qu,Zongyi Yi,Shiyou Zhu,Chunhui Wang,Zhongzheng Cao,Zhuo Zhou,Pengfei Yuan,Ying Yu,Feng Tian,Zhiheng Liu,Ying Bao,Yanxia Zhao,Wensheng Wei +12 more
TL;DR: This work presents an approach, called leveraging endogenous ADAR for programmable editing of RNA (LEAPER), that employs short engineered ADAR-recruiting RNAs (arRNAs) to recruit native ADAR1 or ADAR2 enzymes to change a specific adenosine to inosine.
References
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Journal ArticleDOI
The plant immune system
TL;DR: A detailed understanding of plant immune function will underpin crop improvement for food, fibre and biofuels production and provide extraordinary insights into molecular recognition, cell biology and evolution across biological kingdoms.
Journal ArticleDOI
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
TL;DR: A novel approach for enhancing the accumulation of natural products based on activation tagging by Agrobacterium-mediated transformation with a T-DNA that carries cauliflower mosaic virus 35S enhancer sequences at its right border is reported.
Journal ArticleDOI
Innate immunity in plants : an arms race between pattern recognition receptors in plants and effectors in microbial pathogens
Thomas Boller,Sheng Yang He +1 more
TL;DR: It turns out that the important contribution of PTI to disease resistance is masked by pathogen virulence effectors that have evolved to suppress it.
Journal ArticleDOI
High-frequency modification of plant genes using engineered zinc-finger nucleases
Jeffrey A. Townsend,David A. Wright,Ronnie J. Winfrey,Fengli Fu,Morgan L. Maeder,J. Keith Joung,Daniel F. Voytas +6 more
TL;DR: High-frequency ZFN-stimulated gene targeting at endogenous plant genes, namely the tobacco acetolactate synthase genes (ALS SuRA and SuRB), for which specific mutations are known to confer resistance to imidazolinone and sulphonylurea herbicides are demonstrated.
Journal ArticleDOI
A bacterial effector acts as a plant transcription factor and induces a cell size regulator.
TL;DR: It is shown that AvrBs3 induces the expression of a master regulator of cell size, upa20, which encodes a transcription factor containing a basic helix-loop-helix domain that provokes developmental reprogramming of host cells by mimicking eukaryotic transcription factors.