Journal ArticleDOI
Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors
Jens Boch,Heidi Scholze,Sebastian Schornack,Angelika Landgraf,Simone Hahn,Sabine Kay,Thomas Lahaye,Anja Nickstadt,Ulla Bonas +8 more
TLDR
The functionality of a distinct type of DNA binding domain is described and allows the design ofDNA binding domains for biotechnology.Abstract:
The pathogenicity of many bacteria depends on the injection of effector proteins via type III secretion into eukaryotic cells in order to manipulate cellular processes. TAL (transcription activator-like) effectors from plant pathogenic Xanthomonas are important virulence factors that act as transcriptional activators in the plant cell nucleus, where they directly bind to DNA via a central domain of tandem repeats. Here, we show how target DNA specificity of TAL effectors is encoded. Two hypervariable amino acid residues in each repeat recognize one base pair in the target DNA. Recognition sequences of TAL effectors were predicted and experimentally confirmed. The modular protein architecture enabled the construction of artificial effectors with new specificities. Our study describes the functionality of a distinct type of DNA binding domain and allows the design of DNA binding domains for biotechnology.read more
Citations
More filters
Journal ArticleDOI
Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.
George H. Silva,Laurent Poirot,Roman Galetto,Julianne Smith,Guillermo Montoya,Philippe Duchateau,Frédéric Paques +6 more
TL;DR: These alternative approaches based on non-viral vectorization and/or targeted insertion aimed at achieving safer gene transfer are reviewed, with a special emphasis on megan nucleases, a family of naturally occurring rare-cutting endonucleases, and speculate on their current and future potential.
Journal ArticleDOI
A Receptor Pair with an Integrated Decoy Converts Pathogen Disabling of Transcription Factors to Immunity
Clémentine Le Roux,Clémentine Le Roux,Clémentine Le Roux,Gaëlle Huet,Gaëlle Huet,Alain Jauneau,Laurent Camborde,Laurent Camborde,Dominique Tremousaygue,Dominique Tremousaygue,Alexandra Kraut,Alexandra Kraut,Binbin Zhou,Binbin Zhou,Marie Levaillant,Marie Levaillant,Hiroaki Adachi,Hirofumi Yoshioka,Sylvain Raffaele,Sylvain Raffaele,Richard Berthomé,Richard Berthomé,Yohann Couté,Yohann Couté,Jane E. Parker,Laurent Deslandes,Laurent Deslandes +26 more
TL;DR: It is shown that PopP2 directly acetylates a key lysine within an additional C-terminal WRKY transcription factor domain of RRS1-R that binds DNA, causing loss of WRKY-DNA binding and transactivating functions needed for defense gene expression and disease resistance.
Journal ArticleDOI
Gene targeting by the TAL effector PthXo2 reveals cryptic resistance gene for bacterial blight of rice
Junhui Zhou,Zhao Peng,Juying Long,Juying Long,Davide Sosso,Bo Liu,Joon-Seob Eom,Sheng Huang,Sanzhen Liu,Casiana Vera Cruz,Wolf B. Frommer,Frank F. White,Bing Yang +12 more
TL;DR: The identification of the sucrose transporter gene OsSWEET13 as the disease-susceptibility gene for PthXo2 and the existence of cryptic recessive resistance to Pth Xo2-dependent X. oryzae pv.
Journal ArticleDOI
Expanding the genetic editing tool kit: ZFNs, TALENs, and CRISPR-Cas9.
Rajat M. Gupta,Kiran Musunuru +1 more
TL;DR: This review will outline the recent development of genome-editing technology, culminating with the use of CRISPR-Cas9 to generate novel mammalian models of disease.
Journal ArticleDOI
Regulation of selected genome loci using de novo-engineered transcription activator-like effector (TALE)-type transcription factors
TL;DR: The data demonstrate that the TALE scaffold can be tailored to target user-defined DNA sequences in whole genomes and mediates specific interaction with G nucleotides that thus far could not be targeted specifically by any known RVD type.
References
More filters
Journal ArticleDOI
The plant immune system
TL;DR: A detailed understanding of plant immune function will underpin crop improvement for food, fibre and biofuels production and provide extraordinary insights into molecular recognition, cell biology and evolution across biological kingdoms.
Journal ArticleDOI
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
TL;DR: A novel approach for enhancing the accumulation of natural products based on activation tagging by Agrobacterium-mediated transformation with a T-DNA that carries cauliflower mosaic virus 35S enhancer sequences at its right border is reported.
Journal ArticleDOI
Innate immunity in plants : an arms race between pattern recognition receptors in plants and effectors in microbial pathogens
Thomas Boller,Sheng Yang He +1 more
TL;DR: It turns out that the important contribution of PTI to disease resistance is masked by pathogen virulence effectors that have evolved to suppress it.
Journal ArticleDOI
High-frequency modification of plant genes using engineered zinc-finger nucleases
Jeffrey A. Townsend,David A. Wright,Ronnie J. Winfrey,Fengli Fu,Morgan L. Maeder,J. Keith Joung,Daniel F. Voytas +6 more
TL;DR: High-frequency ZFN-stimulated gene targeting at endogenous plant genes, namely the tobacco acetolactate synthase genes (ALS SuRA and SuRB), for which specific mutations are known to confer resistance to imidazolinone and sulphonylurea herbicides are demonstrated.
Journal ArticleDOI
A bacterial effector acts as a plant transcription factor and induces a cell size regulator.
TL;DR: It is shown that AvrBs3 induces the expression of a master regulator of cell size, upa20, which encodes a transcription factor containing a basic helix-loop-helix domain that provokes developmental reprogramming of host cells by mimicking eukaryotic transcription factors.