Growing and analyzing static biofilms
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TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Real-time monitoring of mono- and dual-species biofilm formation and eradication using microfluidic platform
V. N. Tran,Fazlurrahman Khan,Won Han,Maknuna Luluil,Van Gia Truong,Hyo Geun Yun,Sungyoung Choi,Young-Mog Kim,Joong Ho Shin,Hyun Wook Kang +9 more
TL;DR: In this article , the authors employed a microfluidic technique to analyze the real-time formation of mono and dual-species (S. aureus and C. albicans) biofilms and noninvasive optical treatment of the established mature biofilm using 405-nm laser light.
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Multiple Dictyostelid Species Destroy Biofilms of Klebsiella oxytoca and Other Gram Negative Species.
Dean Sanders,Katarzyna D. Borys,Fikrullah Kisa,Sheryl A. Rakowski,Marcela Lozano,Marcin Filutowicz +5 more
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Effect of serogroup, surface material and disinfectant on biofilm formation by avian pathogenic Escherichia coli.
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An improvised microtiter dish biofilm assay for non-invasive biofilm detection on microbial fuel cell anodes and studying biofilm growth conditions.
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E-Cigarette Aerosols Promote Oral S. aureus Colonization by Delaying an Immune Response and Bacterial Clearing
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References
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Genetic approaches to study of biofilms
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TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
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