Growing and analyzing static biofilms
TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Journal ArticleDOI
Characteristics of Dogs with Biofilm‐Forming Escherichia Coli Urinary Tract Infections
TL;DR: The potential for biofilm formation should be considered whenever E. coli UTI is diagnosed and the association of antibiotic resistance and biofilm potential may affect treatment of UTIs, but additional investigation is warranted.
Journal ArticleDOI
Burkholderia cepacia Complex Contact-Dependent Growth Inhibition Systems Mediate Interbacterial Competition.
TL;DR: It is demonstrated that a Bcc species, Burkholderia multivorans, produces functional CDI system proteins and that growth of this pathogen can be controlled by CDISystem proteins produced by neighboring Burk holderia cells.
Journal ArticleDOI
Differences in biofilm formation and transcription of biofilm-associated genes among Acinetobacter baumannii clinical strains belonging to the international clone II lineage.
Kozue Kishii,Kozue Kishii,Masakaze Hamada,Kotaro Aoki,Kengo Ito,Joh Onodera,Yoshikazu Ishii,Kazuhiro Tateda +7 more
TL;DR: Transcriptional levels of csu operon genes were highly divergent, with comprehensive analysis indicating that regulatory factors other than bfmS/R were involved in biofilm formation by A. baumannii IC II strains.
Journal ArticleDOI
Sustained Release of a Streptococcus pneumoniae Endolysin from Liposomes for Potential Otitis Media Treatment.
TL;DR: In this paper, the MSlys endolysin was encapsulated in deformable liposomes for a targeted treatment of S. pneumoniae, one of the most important causative agents of otitis media.
Journal ArticleDOI
Design, synthesis, and biodistribution studies of new analogues of marine alkaloids: Potent in vitro and in vivo fungicidal agents against Candida spp.
Jéssica Tauany Andrade,William Gustavo Lima,Jaqueline França Sousa,Aline Aparecida Saldanha,Nívea Pereira de Sá,Fernanda Barbara Morais,Mayra Karla Prates da Silva,Gustavo Henrique Ribeiro Viana,Susana Johann,Adriana Cristina Soares,Leonardo Allan Araújo,Simone Odília Antunes Fernandes,Valbert Nascimento Cardoso,Jaqueline Maria Siqueira Ferreira +13 more
TL;DR: The in-vitro anti-Candida activity of four alkaloid synthetic derivatives and their antifungal potential in a murine model of IAC showed that the compound 6a is a potent anti- Candida agent in Vitro and in vivo.
References
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Book ChapterDOI
Genetic approaches to study of biofilms
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TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
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Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
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