Growing and analyzing static biofilms
TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Book ChapterDOI
Nanotechnology and Functional Food
Abhishek Mandal,Ena Ray Banerjee +1 more
TL;DR: This chapter highlights the systematic approach which can be undertaken in studying the dynamics of commensal-immune cell-structural intestinal relations in food industries as well as in food technology.
Journal ArticleDOI
Antibiofilm Efficacy of the Pseudomonas aeruginosa Pbunavirus vB_PaeM-SMS29 Loaded onto Dissolving Polyvinyl Alcohol Microneedles
TL;DR: Overall, this study shows that the distribution of phages caused by the mechanical disruption of biofilms using dissolving microneedles can be an effective delivery method against topical biofilm-related skin infections.
Journal ArticleDOI
The Pseudomonas aeruginosa DksA1 protein is involved in H2O2 tolerance and within-macrophages survival and can be replaced by DksA2
Alessandra Fortuna,Di Collalto,Veronica Schiaffi,Valentina Pastore,Paolo Visca,Fiorentina Ascenzioni,Giordano Rampioni,Livia Leoni +7 more
TL;DR: In this article , the role of DksA paralogs in P. aeruginosa tolerance to H 2 O 2 -induced oxidative stress has been investigated, and it was shown that the DKSA-dependent tolerance to oxidative stress involves, at least in part, the positive transcriptional control of both katA and katE catalase-encoding genes.
Journal ArticleDOI
Basic physiology of Pseudomonas aeruginosa contacted with carbon nanocomposites
Adrian Augustyniak,Kamila Dubrowska,Joanna Jabłońska,Krzysztof Cendrowski,Rafał J. Wróbel,Mateusz Piz,Elzbieta Filipek,Rafał Rakoczy +7 more
TL;DR: In this article , a series of experiments describing the basic physiology of Pseudomonas aeruginosa were performed on selected carbon nanomaterials and their components and the results revealed changes in respiration and growth dynamics that can be used to search for nanocomposites' application in biotechnology.
Journal ArticleDOI
Sucrose selectively regulates Streptococcus mutans polysaccharide by GcrR.
Hongyu Zhang,Mengying Xia,Bin Zhang,Yueling Zhang,Hong Hui Chen,Yalan Deng,Yingming Yang,Lei Lei,Tao Hu +8 more
TL;DR: GcrR interacts with VicR to block EPS biosynthesis via polysaccharide digestion by DexA, and that this process is induced in a sucrose-selective manner, revealing GcrR is a potential strategy for the management of dental caries.
References
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Journal ArticleDOI
Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices.
Gordon D. Christensen,W A Simpson,J J Younger,Larry M. Baddour,F F Barrett,D M Melton,E H Beachey +6 more
TL;DR: The optical densities of stained bacterial films adherent to plastic tissue culture plates serve as a quantitative model for the study of the adherence of coagulase-negative staphylococci to medical devices, a process which may be important in the pathogenesis of foreign body infections.
Journal ArticleDOI
Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin
Marshall C. Walters Iii,Frank L. Roe,Amandine Bugnicourt,Michael J. Franklin,Michael J. Franklin,Philip S. Stewart +5 more
TL;DR: Results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.
Journal ArticleDOI
Role of Antibiotic Penetration Limitation in Klebsiella pneumoniae Biofilm Resistance to Ampicillin and Ciprofloxacin
TL;DR: The results suggest that some other resistance mechanism is involved for both agents and contributed to wild-type biofilm resistance to ampicillin but not to ciprofloxacin.
Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
Journal ArticleDOI
Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
TL;DR: The results demonstrate that the mutants were impaired in the accumulative phase of biofilm production by S. epidermidis by mediating intercellular adhesion.