Growing and analyzing static biofilms
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TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Journal ArticleDOI
Exogenous Protein as an Environmental Stimulus of Biofilm Formation in Select Bacterial Strains
Donna Ye,Lekha Bapu,Mariane Mota Cavalcante,Jesse Kato,Maggie Lauria Sneideman,Kim T. Scribner,Thomas P. Loch,Terence L. Marsh +7 more
TL;DR: Information from a screening of environmental conditions that would elicit robust biofilm in a collection of Serratia marcescens isolated from soil revealed that exogenous milk protein increased biofilm productivity up to ten-fold, suggesting that relatively high concentrations of exogenous protein may serve as an environmental trigger for biofilm formation.
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Ranking of major classes of antibiotics for activity against stationary phase Pseudomonas aeruginosa and identification of Clinafloxacin + Cefuroxime + Gentamicin drug combination that eradicates persistent P. aeruginosa infection in a murine cystic fibrosis model
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Soluble chitosan derivative treats wound infections and promotes wound healing in a novel MRSA-infected porcine partial-thickness burn wound model
Francesco M. Egro,Alexander J. Repko,Vidya P. Narayanaswamy,Asim Ejaz,Deokyeol Y. Kim,Mark A. Schusterman,Allister J. Loughran,Ali M. Ayyash,Stacy M Towsend,Shenda M. Baker,Jenny A. Ziembicki,Kacey G. Marra,Peter Rubin +12 more
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Transgenesis of mammalian PABP reveals mRNA polyadenylation as a general stress response mechanism in bacteria.
TL;DR: In this article, a trans-expression of the mammalian nuclear PABPN1 chromosomally and extra-chromosomally in Escherichia coli was shown to improve the half-life of polyadenylated mRNAs.
References
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Journal ArticleDOI
Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices.
Gordon D. Christensen,W A Simpson,J J Younger,Larry M. Baddour,F F Barrett,D M Melton,E H Beachey +6 more
TL;DR: The optical densities of stained bacterial films adherent to plastic tissue culture plates serve as a quantitative model for the study of the adherence of coagulase-negative staphylococci to medical devices, a process which may be important in the pathogenesis of foreign body infections.
Journal ArticleDOI
Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin
Marshall C. Walters Iii,Frank L. Roe,Amandine Bugnicourt,Michael J. Franklin,Michael J. Franklin,Philip S. Stewart +5 more
TL;DR: Results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.
Journal ArticleDOI
Role of Antibiotic Penetration Limitation in Klebsiella pneumoniae Biofilm Resistance to Ampicillin and Ciprofloxacin
TL;DR: The results suggest that some other resistance mechanism is involved for both agents and contributed to wild-type biofilm resistance to ampicillin but not to ciprofloxacin.
Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
Journal ArticleDOI
Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
TL;DR: The results demonstrate that the mutants were impaired in the accumulative phase of biofilm production by S. epidermidis by mediating intercellular adhesion.