Growing and analyzing static biofilms
TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Journal ArticleDOI
Phages from Ganges River curtail in vitro biofilms and planktonic growth of drug resistant Klebsiella pneumoniae in a zebrafish infection model.
Niranjana Sri Sundaramoorthy,Subramanian Thothathri,Muthu Meenakshi Bhaskaran,ArunKumar GaneshPrasad,Saisubramanian Nagarajan +4 more
TL;DR: In this paper, the authors evaluated phage populations from water bodies to inhibit planktonic and biofilm mode of growth of drug resistant Klebsiella pneumoniae in vitro and curtail PL growth in vivo in a zebrafish model.
Journal ArticleDOI
Comparative Transcriptomic and Functional Assessments of Linezolid-Responsive Small RNA Genes in Staphylococcus aureus
Wei Gao,Romain Guérillot,Ya Hsun Lin,Jai J. Tree,Marie-Emilie Beaume,Patrice Francois,Ian R. Monk,Torsten Seemann,Jacques Schrenzel,Benjamin P Howden,Timothy P. Stinear +10 more
TL;DR: The data suggest sRNA functional redundancy, because despite their high expression levels upon antibiotic exposure, individual s RNA genes do not affect readily observable bacterial phenotypes, and underscore the need for sensitive assays and new approaches to try and decipher the functions of sRNA genes in S. aureus.
Journal ArticleDOI
Similar virulence properties of infection and colonization associated Pseudomonas aeruginosa.
TL;DR: ExoU positivity is associated with severe disease, as evidenced by the longer duration of hospital stay of these patients, but the presence of virulence factors or multi‐drug resistance in the cultured strain should not prompt the administration of anti‐pseudomonal chemotherapy.
Journal ArticleDOI
Impedance spectroscopic study of biofilm formation on pencil lead graphite anode in microbial fuel cell
Rahul Kandpal,Sharda Nara,Mohammad Shahadat,Mohammad Shahadat,Mohammad Omaish Ansari,Ahmed Alshahrie,Syed Wazed Ali,Syed Wazed Ali,Shaikh Ziauddin Ahammad,Shaikh Ziauddin Ahammad,Bansi D. Malhotra +10 more
TL;DR: In this paper, the formation of Bacillus subtilis 168 biofilm on a pencil lead graphite was assessed in an MFC using electrochemical impedance spectroscopy (EIS).
Journal ArticleDOI
Mechanisms underlying interactions between two abundant oral commensal bacteria.
Dasith Perera,Anthony R. McLean,Viviana Morillo-Lopez,Kaileigh Cloutier-Leblanc,Eric Almeida,Kiana Cabana,Jessica L. Mark Welch,Matthew Ramsey +7 more
TL;DR: The authors found that H. parainfluenzae utilizes a more redundant, multifactorial response to H2O2 than related microorganisms and that this system's integrity enhances streptococcal fitness.
References
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Genetic approaches to study of biofilms
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