Growing and analyzing static biofilms
TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
More filters
Book ChapterDOI
Methods for biofilm constituents and turnover
Arnaud Bridier,Florence Dubois-Brissonnet,Romain Briandet,Clayton E. Cox,Clayton E. Cox,William J. Zaragoza,William J. Zaragoza,Cory J. Krediet,Cory J. Krediet,Max Teplitski,Max Teplitski +10 more
Journal ArticleDOI
Nano- and Macroscale Imaging of Cholesterol Linoleate and Human Beta Defensin 2-Induced Changes in Pseudomonas aeruginosa Biofilms
Brent Beadell,Andy Chieng,Kevin R. Parducho,Zhipeng Dai,Sam On Ho,Gary Fujii,Yixian Wang,Edith Porter +7 more
TL;DR: In this paper, a phospholipid-based liposome formulation of cholesteryl lineolate (CL-PL) was applied to Pseudomonas aeruginosa (PA) biofilm formation.
Journal ArticleDOI
Establishment of a Novel Rat Model of Gram-Negative Periprosthetic Joint Infection Using Cementless Hip Hemiarthroplasty
TL;DR: In this paper , a clinically representative periprosthetic joint infection (GN-PJI) model that recapitulates biofilm formation in vivo was established, and implant stability was directly assessed by quantifying osseointegration using microcomputed tomography, and indirectly assessed by identifying gait-pattern changes.
Evaluation of Biofouling and Its Control by Hypochlorite on Polyamide Reverse Osmosis Membranes
TL;DR: In this paper, the authors evaluated biofouling potential by direct analysis of RO membrane through fluorescence intensity analysis of biofilm formed on the membrane surface, thereby incorporating fouling tendencies of both feedwater and membrane.
References
More filters
Journal ArticleDOI
Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices.
Gordon D. Christensen,W A Simpson,J J Younger,Larry M. Baddour,F F Barrett,D M Melton,E H Beachey +6 more
TL;DR: The optical densities of stained bacterial films adherent to plastic tissue culture plates serve as a quantitative model for the study of the adherence of coagulase-negative staphylococci to medical devices, a process which may be important in the pathogenesis of foreign body infections.
Journal ArticleDOI
Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin
Marshall C. Walters Iii,Frank L. Roe,Amandine Bugnicourt,Michael J. Franklin,Michael J. Franklin,Philip S. Stewart +5 more
TL;DR: Results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.
Journal ArticleDOI
Role of Antibiotic Penetration Limitation in Klebsiella pneumoniae Biofilm Resistance to Ampicillin and Ciprofloxacin
TL;DR: The results suggest that some other resistance mechanism is involved for both agents and contributed to wild-type biofilm resistance to ampicillin but not to ciprofloxacin.
Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
Journal ArticleDOI
Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
TL;DR: The results demonstrate that the mutants were impaired in the accumulative phase of biofilm production by S. epidermidis by mediating intercellular adhesion.