Growing and analyzing static biofilms
TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Journal ArticleDOI
Influence of Probiotic Culture Supernatants on In Vitro Biofilm Formation of Staphylococci.
TL;DR: The inhibitory effects of the S. salivarius K12 culture supernatant on the formation of staphylococcal biofilms are of potential relevance for biofilm-associated diseases and should be further assessed by in vivo infection models.
Journal ArticleDOI
The effect of some environmental conditions on planktonic growth and biofilm formation by some lactic acid bacteria isolated from a local cheese in Turkey
TL;DR: It was determined that lactic acid bacteria, which were previously determined to have some starter culture characteristics, had additional properties on the way to being an starter culture.
Journal ArticleDOI
Characterization, antibiofilm and biocompatibility properties of chitosan hydrogels loaded with silver nanoparticles and ampicillin: an alternative protection to central venous catheters.
Montserrat Lopez-Carrizales,Esmeralda Mendoza-Mendoza,René D. Peralta-Rodríguez,Mario Alberto Pérez-Díaz,Diana P. Portales-Pérez,Martín Magaña-Aquino,Antonio Aragón-Piña,Ramiro Infante-Martínez,Enrique Díaz Barriga-Castro,Roberto Sánchez-Sánchez,Gabriel Alejandro Martínez-Castañón,Fidel Martinez-Gutierrez +11 more
TL;DR: Results suggest that AgNPs and AMP CHs loaded could be used as prophylactic treatment in patients with central venous catheter (CVC), inhibiting the formation of biofilms in their early stages, in addition to their anticoagulant effect and biocompatibility.
Journal ArticleDOI
Effect of Biofilm Formation by Lactobacillus plantarum on the Malolactic Fermentation in Model Wine.
Gianfranco Pannella,Silvia Jane Lombardi,Francesca Coppola,Franca Vergalito,Massimo Iorizzo,Mariantonietta Succi,Patrizio Tremonte,Caterina Iannini,Elena Sorrentino,Raffaele Coppola +9 more
TL;DR: Cell in biofilm life-style showed high resistance, assuring a prompt and complete L-malic acid conversion in wine stress conditions and highlighted that, regardless of isolation source and stress conditions, the ability to form biofilm was strain-dependent.
Journal ArticleDOI
Antibiofilm effect of supramolecularly templated mesoporous silica coatings.
Magdalena Pezzoni,Paolo N. Catalano,Ramón A. Pizarro,Martín F. Desimone,Galo J. A. A. Soler-Illia,Martín G. Bellino,Cristina S. Costa +6 more
TL;DR: Assays employing submerged and air-liquid interface biofilms demonstrated that mesoporous coatings with tuned pore size significantly reduce the number of attached bacteria and matrix production.
References
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TL;DR: Results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.
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Role of Antibiotic Penetration Limitation in Klebsiella pneumoniae Biofilm Resistance to Ampicillin and Ciprofloxacin
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Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
Journal ArticleDOI
Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
TL;DR: The results demonstrate that the mutants were impaired in the accumulative phase of biofilm production by S. epidermidis by mediating intercellular adhesion.