Growing and analyzing static biofilms
TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
More filters
Journal ArticleDOI
Pseudomonas aeruginosa Biofilm Antibiotic Resistance Gene ndvB Expression Requires the RpoS Stationary-Phase Sigma Factor.
Clayton W. Hall,Aaron Hinz,Luke B.-P. Gagnon,Li Zhang,Jean-Paul Nadeau,Sarah J. Copeland,Bratati Saha,Thien-Fah Mah +7 more
TL;DR: The results support the physiological similarity between biofilms and stationary-phase cells and suggest that the induction of some stationary- phase genes in biofilm may contribute to their increased antibiotic resistance.
Journal ArticleDOI
Prevention of polymicrobial biofilms composed of Pseudomonas aeruginosa and pathogenic fungi by essential oils from selected Citrus species.
Marina Pekmezovic,Ivana Aleksic,Aleksandra Barac,Valentina Arsic-Arsenijevic,Branka Vasiljevic,Jasmina Nikodinovic-Runic,Lidija Senerovic +6 more
TL;DR: Pompia and grapefruit EOs potently inhibited biofilm formation and could be used for the control of common polymicrobial infections.
Journal ArticleDOI
Antibiofilm, antiquorum sensing and antioxidant activity of secondary metabolites from seeds of Annona senegalensis, Persoon
Alfred Ngenge Tamfu,Ozgur Ceylan,Godloves Chi Fru,Mehmet Öztürk,Mehmet Emin Duru,Farzana Shaheen +5 more
TL;DR: The increasing resistance of bacteria to antibiotics has motivated the interest in potent natural compounds capable of disrupting bacterial cell-to-cell communication, including N-cerotoyltryptamine, asimicin, and ent-19-carbomethoxykauran-17-oic acid, which were tested for their antimicrobial, antibiofilm, and anti-quorum sensing activities.
Journal ArticleDOI
Genomic, morphological and functional characterisation of novel bacteriophage FNU1 capable of disrupting Fusobacterium nucleatum biofilms.
Mwila Kabwe,Teagan L. Brown,Stuart G. Dashper,Lachlan B. M. Speirs,Heng Ku,Steve Petrovski,Hiu Tat Chan,Peter Lock,Joseph Tucci +8 more
TL;DR: The morphology, genomics and functional characteristics of FNU1, a novel bacteriophage lytic against F. nucleatum were described and it was found to be a large Siphoviridae virus with capsid diameter and tail, and its genome was 130914 bp.
Journal ArticleDOI
Sporulation dynamics and spore heat resistance in wet and dry biofilms of Bacillus cereus
TL;DR: The results indicate that environmental conditions encountered by bio Films affect sporulation dynamics and spore heat resistance, thus affecting subsequent quality issues and safety risks related to these biofilms.
References
More filters
Journal ArticleDOI
Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices.
Gordon D. Christensen,W A Simpson,J J Younger,Larry M. Baddour,F F Barrett,D M Melton,E H Beachey +6 more
TL;DR: The optical densities of stained bacterial films adherent to plastic tissue culture plates serve as a quantitative model for the study of the adherence of coagulase-negative staphylococci to medical devices, a process which may be important in the pathogenesis of foreign body infections.
Journal ArticleDOI
Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin
Marshall C. Walters Iii,Frank L. Roe,Amandine Bugnicourt,Michael J. Franklin,Michael J. Franklin,Philip S. Stewart +5 more
TL;DR: Results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.
Journal ArticleDOI
Role of Antibiotic Penetration Limitation in Klebsiella pneumoniae Biofilm Resistance to Ampicillin and Ciprofloxacin
TL;DR: The results suggest that some other resistance mechanism is involved for both agents and contributed to wild-type biofilm resistance to ampicillin but not to ciprofloxacin.
Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
Journal ArticleDOI
Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
TL;DR: The results demonstrate that the mutants were impaired in the accumulative phase of biofilm production by S. epidermidis by mediating intercellular adhesion.