Growing and analyzing static biofilms
TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Journal ArticleDOI
Recycled desalination membranes as a support material for biofilm development: A new approach for microcystin removal during water treatment.
Jesús Morón-López,Jesús Morón-López,Lucía Nieto-Reyes,Jorge Senán-Salinas,Serena Molina,Rehab El-Shehawy,Rehab El-Shehawy +6 more
TL;DR: This multidisciplinary work showed that the inherent flaw of RO membranes, i.e., fouling, can be considered an advantageous characteristic for biofilm attachment and could be a future green cost-effective alternative technology for MC removal.
Journal ArticleDOI
Antimicrobial activity of Amazon Astrocaryum aculeatum extracts and its association to oxidative metabolism.
Micheli Lamberti Jobim,Roberto Christ Vianna Santos,Camilla Filippi dos Santos Alves,Raul Moreira Oliveira,Clarice Pinheiro Mostardeiro,Michele Rorato Sagrillo,Olmiro Cezimbra de Souza Filho,Luiz Filipe Machado Garcia,Maria Fernanda Manica-Cattani,Euler Esteves Ribeiro,Ivana Beatrice Mânica da Cruz +10 more
TL;DR: Tucumã extracts present antimicrobial activity to four microorganisms that have large problems of drug resistance, and the possible mechanism of action of this Amazon fruit is related to REDOX imbalance.
Journal ArticleDOI
A Preliminary Study of Chemical Profiles of Honey, Cerumen, and Propolis of the African Stingless Bee Meliponula ferruginea.
Milena Popova,Dessislava Gerginova,Boryana Trusheva,Svetlana Simova,Alfred Ngenge Tamfu,Ozgur Ceylan,Kerry Clark,Vassya Bankova +7 more
TL;DR: In this paper, the chemical composition of honey, two cerumen, and two resin deposits (propolis) samples of Meliponula ferruginea from Tanzania were analyzed by NMR and indicated different long-term stability from Apis mellifera European (Bulgarian) honey.
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Characterization of lasR-deficient clinical isolates of Pseudomonas aeruginosa
Yao Wang,Leiqiong Gao,Leiqiong Gao,Xiancai Rao,Jing Wang,Hua Yu,Junru Jiang,Wei Zhou,Jin Wang,Yonghong Xiao,Mengwen Li,Yan Zhang,Kebin Zhang,Li Shen,Ziyu Hua +14 more
TL;DR: The hypothesis that conventional QS hierarchy can be smashed by naturally occurring lasR mutation in clinical P. aeruginosa isolates is supported and that complex QS hierarchies may play a role in maintaining infection of this opportunistic pathogen.
Journal ArticleDOI
An Oxygen-Sensing Two-Component System in the Burkholderia cepacia Complex Regulates Biofilm, Intracellular Invasion, and Pathogenicity.
Matthew M. Schaefers,Tiffany L. Liao,Nicole M. Boisvert,Damien Roux,Deborah R. Yoder-Himes,Gregory P. Priebe,Gregory P. Priebe +6 more
TL;DR: B. dolosa fixLJ is a global regulator that controls biofilm formation, motility, intracellular invasion/persistence, and virulence and is found to be less invasive in human lung epithelial and macrophage-like cells.
References
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