Growing and analyzing static biofilms
TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Journal ArticleDOI
Tetrasodium EDTA Is Effective at Eradicating Biofilms Formed by Clinically Relevant Microorganisms from Patients' Central Venous Catheters.
Fangning Liu,Satyender Hansra,Gordon Crockford,Wolfgang Köster,Wolfgang Köster,Brenda Allan,Joseph M Blondeau,Joseph M Blondeau,Chantal Lainesse,Aaron White +9 more
TL;DR: Tetrasodium EDTA was effective at eliminating Gram-positive, Gram-negative, and fungal species and represents a promising alternative to antibiotic treatment with less chance of the organisms developing resistance, and fits with antibiotic stewardship programs worldwide by sparing the use of important antibiotic agents.
Journal ArticleDOI
Regulation of water-soluble glucan synthesis by the Streptococcus mutans dexA gene effects biofilm aggregation and cariogenic pathogenicity.
Yan Yang,Yan Yang,Mengying Mao,Lei Lei,Meng Li,Jia-Xin Yin,Xin-Rong Ma,Xiang Tao,Yingming Yang,Tao Hu +9 more
TL;DR: The results show that deletion of dexA (SmudexA) results in increased transcription of EPS synthesis-related genes, including gtfB, gtfD, and ftf, and interestingly, inactivating the dexA gene may lead to elevated WSG synthesis in S mutans, which results in dysregulated cariogenicity in vivo.
Journal ArticleDOI
Identification and characterization of novel glycoproteins involved in growth and biofilm formation by Porphyromonas gingivalis
M. Kishi,Yoshiaki Hasegawa,Yoshiaki Hasegawa,Keiji Nagano,Hiroshi Nakamura,Yukitaka Murakami,Fuminobu Yoshimura +6 more
TL;DR: Results suggest that the novel glycoproteins PGN0743 and PGN0876 play an important role in the growth and colonization of P. gingivalis.
Journal ArticleDOI
Discrimination of bacteriophage infected cells using locked nucleic acid fluorescent in situ hybridization (LNA-FISH)
Diana Patrícia Andrade Vilas Boas,Carina Almeida,Sanna Sillankorva,Ana Nicolau,Joana Azeredo,Nuno F. Azevedo +5 more
TL;DR: In biofilm structures, LNA-FISH provided a good discrimination of the infected cells and also allowed the assessment of the spatial distribution of infected and non-infected populations.
Journal ArticleDOI
Analysis of Clostridium difficile biofilms: imaging and antimicrobial treatment.
Garth A. James,Laurent Chesnel,Laura Boegli,Elinor deLancey Pulcini,Steve T. Fisher,Philip S. Stewart +5 more
TL;DR: Fidaxomicin is effective in disrupting C. difficile biofilms, killing vegetative cells and decreasing spore counts, compared with vancomycin or metronidazole.
References
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Genetic approaches to study of biofilms
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