Growing and analyzing static biofilms
TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Posted ContentDOI
OprF impacts Pseudomonas aeruginosa biofilm matrix eDNA levels in a nutrient-dependent manner
Erin K. Cassin,Sophia A. Araujo-Hernandez,Dena S. Baughn,Melissa C. Londono,Daniela Q. Rodriguez,Boo Shan Tseng +5 more
TL;DR: In this paper , the authors identify a nutrient-dependent effect of OprF in static biofilms, whereby ΔoprF cells form significantly less biofilm than wild type when grown in media containing glucose or low sodium chloride concentrations.
Journal Article
Experimental biofilms within drinking water treatment plant origin; evaluation of nutrient concentration and temperature influences upon their development.
TL;DR: This exploratory experiment was performed in order to investigate the influence of certain environmental factors such as nutrient concentration and temperature upon in vitro biofilm’s development, origin in the biofilm of water treatment plant.
Journal ArticleDOI
Csp1, a Cold Shock Protein Homolog in Xylella fastidiosa Influences Cell Attachment, Pili Formation, and Gene Expression.
TL;DR: In this paper, the role of cold shock protein Csp1 in X. fastidiosa virulence was investigated by comparing the wild and Δcsp1 deletion mutants, which showed reduced cell-to-cell attachment and reduced attachment to surfaces compared to those with the wild type.
Book ChapterDOI
Application of Klebsiella pneumoniae in Treatment and Electricity Generation from Piggery Solid Wastes
Journal ArticleDOI
High-throughput screening alternative to crystal violet biofilm assay combining fluorescence quantification and imaging
TL;DR: In this article, the Cytation 5 multi-mode plate reader was used to enable simultaneous acquisition of both quantitative and imaging biofilm data, which produces more robust data and provides information about morphology and spatial species organization within the biofilm.
References
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Journal ArticleDOI
Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices.
Gordon D. Christensen,W A Simpson,J J Younger,Larry M. Baddour,F F Barrett,D M Melton,E H Beachey +6 more
TL;DR: The optical densities of stained bacterial films adherent to plastic tissue culture plates serve as a quantitative model for the study of the adherence of coagulase-negative staphylococci to medical devices, a process which may be important in the pathogenesis of foreign body infections.
Journal ArticleDOI
Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin
Marshall C. Walters Iii,Frank L. Roe,Amandine Bugnicourt,Michael J. Franklin,Michael J. Franklin,Philip S. Stewart +5 more
TL;DR: Results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.
Journal ArticleDOI
Role of Antibiotic Penetration Limitation in Klebsiella pneumoniae Biofilm Resistance to Ampicillin and Ciprofloxacin
TL;DR: The results suggest that some other resistance mechanism is involved for both agents and contributed to wild-type biofilm resistance to ampicillin but not to ciprofloxacin.
Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
Journal ArticleDOI
Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
TL;DR: The results demonstrate that the mutants were impaired in the accumulative phase of biofilm production by S. epidermidis by mediating intercellular adhesion.