Growing and analyzing static biofilms
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TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Posted ContentDOI
Inhibition of adhesive nanofibrillar mediated Streptococcus gordonii - Candida albicans mono- and dual-species biofilms
TL;DR: Results suggest that S. gordonii stimulates expression of adhesive materials in C. albicans by direct interaction and or by signaling mechanism(s), and these mechanisms can be inhibited by GAs, and further studies on global gene expression of these biofilms and their biochemical studies may reveal the molecular mechanism of their inhibition.
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Computational design of antimicrobial active surfaces via automated Bayesian optimization
Hanfeng Zhai,Jingjie Yeo +1 more
TL;DR: The framework successfully generated ideal nano-surfaces for biofilm removal through applied shear and vibration and provides insights into various engineering fields that require surface-mediatedBiofllm control and can be applied to more general materials design and optimization.
Journal ArticleDOI
Phenotypic and integrated analysis of a comprehensive Pseudomonas aeruginosa PAO1 library of mutants lacking cyclic-di-GMP-related genes
Kira Eilers,Joey Kuok Hoong Yam,Richard A. Morton,Adeline Mei Hui Yong,Jaime Brizuela,Corina Hadjicharalambous,Xianghui Liu,Michael Givskov,Scott A. Rice,Alain Filloux +9 more
TL;DR: A mutant library for the 41 individual dgc/pde genes in P. aeruginosa PAO1 is engineered and a significant to slight variation is observed in the global c-di-GMP pool of cells grown planktonically, while several mutants display a phenotypic impact on biofilm including initial attachment and maturation.
A Cold Atmospheric Pressure Plasma Discharge Device Exerts Antimicrobial Effects.
Kenneth A. Cornell,Kate Benfield,Tiffany Berntsen,Jenna Clingerman,Adam Croteau,Spencer Goering,Daniel Moyer,Mariah Provost,Amanda White,Donald Plumlee,Julia Thom Oxford,Jim Browning +11 more
TL;DR: A cold atmospheric pressure plasma device was developed using two parallel plates of Low Temperature Co-fired Ceramic with embedded electrodes and it was shown that short plasma treatments rapidly killed biofilm resident bacteria with ED90 values of <15 s.
References
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Adherence of coagulase-negative staphylococci to plastic tissue culture plates: a quantitative model for the adherence of staphylococci to medical devices.
Gordon D. Christensen,W A Simpson,J J Younger,Larry M. Baddour,F F Barrett,D M Melton,E H Beachey +6 more
TL;DR: The optical densities of stained bacterial films adherent to plastic tissue culture plates serve as a quantitative model for the study of the adherence of coagulase-negative staphylococci to medical devices, a process which may be important in the pathogenesis of foreign body infections.
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Contributions of Antibiotic Penetration, Oxygen Limitation, and Low Metabolic Activity to Tolerance of Pseudomonas aeruginosa Biofilms to Ciprofloxacin and Tobramycin
Marshall C. Walters Iii,Frank L. Roe,Amandine Bugnicourt,Michael J. Franklin,Michael J. Franklin,Philip S. Stewart +5 more
TL;DR: Results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.
Journal ArticleDOI
Role of Antibiotic Penetration Limitation in Klebsiella pneumoniae Biofilm Resistance to Ampicillin and Ciprofloxacin
TL;DR: The results suggest that some other resistance mechanism is involved for both agents and contributed to wild-type biofilm resistance to ampicillin but not to ciprofloxacin.
Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
Journal ArticleDOI
Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
TL;DR: The results demonstrate that the mutants were impaired in the accumulative phase of biofilm production by S. epidermidis by mediating intercellular adhesion.