Growing and analyzing static biofilms
TLDR
In this article, the early stages of biofilm formation are examined using static biofilm assays, which are suitable for either small or relatively large-scale studies and can be used individually or in combination for the study of biofilms.Abstract:
Many bacteria can exist as surface-attached aggregations known as biofilms. Presented in this unit are several approaches for the study of these communities. The focus here is on static biofilm systems, which are particularly useful for examination of the early stages of biofilm formation, including initial adherence to the surface and microcolony formation. Furthermore, most of the techniques presented are easily adapted to the study of biofilms under a variety of conditions and are suitable for either small- or relatively large-scale studies. Unlike assays involving continuous-flow systems, the static biofilm assays described here require very little specialized equipment and are relatively simple to execute. In addition, these static biofilm systems allow analysis of biofilm formation with a variety of readouts, including microscopy of live cells, macroscopic visualization of stained bacteria, and viability counts. Used individually or in combination, these assays provide useful means for the study of biofilms.read more
Citations
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Journal ArticleDOI
Antimicrobial Orthodontic Wires Coated with Silver Nanoparticles
Isabella Salgado Gonçalves,Ana Beatriz Viale,Natália Navarro Sormani,Karina Eiras Dela Coleta Pizzol,Alyne Rodrigues de Araújo-Nobre,Paula Chagas Silva de Oliveira,Hélida Gomes de Oliveira Barud,Selma Gutierrez Antonio,Hernane da Silva Barud +8 more
TL;DR: In this paper, a green process was used to attach silver nanoparticles (AgNPs) to the surface of two different brands of orthodontic wires and the coated materials were analyzed for their physicochemical properties by scanning electron microscopy (SEM), X-ray diffraction (XRD), and differential scanning calorimetry (DSC).
Journal ArticleDOI
Environmental bacteria produce abundant and diverse antibiofilm compounds.
Jackson T. Farmer,Anton V. Shimkevitch,P.S. Reilly,Kevin D. Mlynek,K.S. Jensen,Mary Theresa Callahan,Karen L. Bushaw-Newton,Jeffrey B. Kaplan +7 more
TL;DR: The aim of this study was to isolate novel antibiofilm compounds produced by environmental bacteria by isolating them from Gram-positive and Gram-negative bacteria.
Journal ArticleDOI
Adhesion to sand and ability to mineralise low pesticide concentrations are required for efficient bioaugmentation of flow-through sand filters
TL;DR: Results demonstrate that besides the ability to degrade the contaminant, surface hydrophobicity and adherence abilities are significant parameters controlling sustained degradation in flow-through sand columns and must be considered when selecting bacteria for bioaugmentation.
Journal ArticleDOI
Staphylococcus Aureus Surface Protein G is An Immunodominant Protein and a Possible Target in An Anti-Biofilm Drug Development.
Yury Belyi,Ivan Rybolovlev,N. B. Polyakov,Alena Chernikova,Irina Tabakova,Alexandre Gintsburg +5 more
TL;DR: Rabbit serum directed against purified recombinant SasG, similar to serum against crude staphylococcal liquid culture, prevented the formation of a biofilm in vitro, indicating that SasG can be considered as a target in an anti-biofilm drug development and a component of the vaccine or immunotherapeutic preparations directed against staphlyococcal infections in humans.
Inhibition of swarming motility, biofilm formation and virulence factor expression of urinary pathogens by euphorbia trigona latex extracts
TL;DR: A remarkable reduction in the swarm zone, virulence factor production and biofilm formation was observed was observed which suggests that Euphorbia latex contains compounds which can potentially be developed as anti pathogenic drugs.
References
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Gordon D. Christensen,W A Simpson,J J Younger,Larry M. Baddour,F F Barrett,D M Melton,E H Beachey +6 more
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Marshall C. Walters Iii,Frank L. Roe,Amandine Bugnicourt,Michael J. Franklin,Michael J. Franklin,Philip S. Stewart +5 more
TL;DR: Results show that oxygen limitation and low metabolic activity in the interior of the biofilm, not poor antibiotic penetration, are correlated with antibiotic tolerance of this P. aeruginosa biofilm system.
Journal ArticleDOI
Role of Antibiotic Penetration Limitation in Klebsiella pneumoniae Biofilm Resistance to Ampicillin and Ciprofloxacin
TL;DR: The results suggest that some other resistance mechanism is involved for both agents and contributed to wild-type biofilm resistance to ampicillin but not to ciprofloxacin.
Book ChapterDOI
Genetic approaches to study of biofilms
George A. O'Toole,Leslie A. Pratt,Paula I. Watnick,Dianne K. Newman,Valerie B. Weaver,Roberto Kolter +5 more
TL;DR: This article operationally defines a biofilm as bacteria that are attached to a surface in sufficient numbers to be detected macroscopically.
Journal ArticleDOI
Characterization of transposon mutants of biofilm-producing Staphylococcus epidermidis impaired in the accumulative phase of biofilm production: genetic identification of a hexosamine-containing polysaccharide intercellular adhesin.
TL;DR: The results demonstrate that the mutants were impaired in the accumulative phase of biofilm production by S. epidermidis by mediating intercellular adhesion.