Journal ArticleDOI
In vivo CD8+ T cell CRISPR screening reveals control by Fli1 in infection and cancer.
Zeyu Chen,Eri Arai,Omar Khan,Zhen Zhang,Shin Foong Ngiow,Yuan He,Hua Huang,Sasikanth Manne,Zhendong Cao,Amy E. Baxter,Zhangying Cai,Elizabeth Freilich,Mohammed Alkhatim A. Ali,Josephine R. Giles,Jennifer E. Wu,Allison R. Greenplate,Mohamed A. Hakeem,Qingzhou Chen,Makoto Kurachi,Kito Nzingha,Viktoriya Ekshyyan,Divij Mathew,Zhuoyu Wen,Nancy A. Speck,Alexis Battle,Shelley L. Berger,E. John Wherry,Junwei Shi +27 more
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TLDR
In this paper, the authors developed an in-vivo T-cell CRISPR screening platform and identified a key mechanism restraining TEFF biology through the ETS family TF, Fli1.About:
This article is published in Cell.The article was published on 2021-03-04. It has received 74 citations till now.read more
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High-content CRISPR screening
TL;DR: Bock et al. as mentioned in this paper described the basic and advanced concepts of CRISPR screening and its application as a flexible and reliable method for biological discovery, biomedical research and drug development, with a special emphasis on high-content methods that make it possible to obtain detailed biological insights directly as part of the screen.
Journal ArticleDOI
High-content CRISPR screening
Christopher J Bock,Paul Datlinger,Florence M. Chardon,Matthew A. Coelho,Matthew B. Dong,Keith A. Lawson,Tian Lu,Laetitia Maroc,Thomas M. Norman,Bicna Song,Geoff Stanley,Sidi Chen,Mathew J. Garnett,Wei Li,Jason Moffat,Lei S. Qi,Rebecca S. Shapiro,Jay Shendure,Jonathan S. Weissman,Xiao-Xi Zhuang +19 more
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A genome-scale screen for synthetic drivers of T cell proliferation
Mateusz Legut,Zoran Gajic,Maria Francesca Guarino,Zharko Daniloski,J. Rahman,Xinhe Xue,Congyi Lu,Lu Lu,Eleni P. Mimitou,Stephanie Hao,Teresa Davoli,Catherine Diefenbach,Peter Smibert,Neville E. Sanjana +13 more
TL;DR: In this paper , the authors identify positive regulators of T cell functions through overexpression of around 12,000 barcoded human open reading frames (ORFs), which increased the proliferation and activation of primary human CD4+ and CD8+ T cells and their secretion of key cytokines such as interleukin-2 and interferonγ.
Journal ArticleDOI
Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence
TL;DR: In this paper , the authors provided an atlas of the genetic regulators of T cell exhaustion and demonstrated that modulation of epigenetic state can improve T cell responses in cancer immunotherapy, which led to improved antitumor immunity.
Journal ArticleDOI
A genome-scale screen for synthetic drivers of T cell proliferation
Mateusz Legut,Zoran Gajic,Maria Francesca Guarino,Zharko Daniloski,J. Rahman,Xinhe Xue,Congyi Lu,Lu Lu,Eleni P. Mimitou,Stephanie Hao,Teresa Davoli,Catherine Diefenbach,Peter Smibert,Neville E. Sanjana +13 more
TL;DR: In this article , the authors identify positive regulators of T cell functions through overexpression of around 12,000 barcoded human open reading frames (ORFs), which increased the proliferation and activation of primary human CD4+ and CD8+ T cells and their secretion of key cytokines such as interleukin-2 and interferonγ.
References
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Journal ArticleDOI
Common pitfalls in preclinical cancer target validation
TL;DR: Some of the common pitfalls in preclinical cancer target identification are outlined and some potential approaches to mitigate them are outlined.
Journal ArticleDOI
Domain-focused CRISPR screen identifies HRI as a fetal hemoglobin regulator in human erythroid cells.
Jeremy D. Grevet,Jeremy D. Grevet,Xianjiang Lan,Nicole Hamagami,Christopher R. Edwards,Laavanya Sankaranarayanan,Xinjun Ji,Saurabh K. Bhardwaj,Carolyne J. Face,David Posocco,Osheiza Abdulmalik,Cheryl A. Keller,Belinda Giardine,Simone Sidoli,Ben A. Garcia,Stella T. Chou,Stephen A. Liebhaber,Ross C. Hardison,Junwei Shi,Gerd A. Blobel +19 more
TL;DR: Results suggest HRI as a potential therapeutic target for hemoglobinopathies, after identifying heme-regulated eIF2α kinase (HRI) as an HbF repressor in adult human RBCs.
Journal ArticleDOI
In vivo CRISPR screening in CD8 T cells with AAV-Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma.
Lupeng Ye,Jonathan J. Park,Matthew B. Dong,Quanjun Yang,Ryan D. Chow,Lei Peng,Yaying Du,Jianjian Guo,Xiaoyun Dai,Guangchuan Wang,Youssef Errami,Sidi Chen +11 more
TL;DR: It is demonstrated that adoptive transfer of CD8+ T cells with Pdia3, Mgat5, Emp1 or Lag3 gene editing enhances the survival of GBM-bearing mice in both syngeneic and T-cell receptor transgenic models.
Journal ArticleDOI
The Transcription Factor Runx3 Establishes Chromatin Accessibility of cis-Regulatory Landscapes that Drive Memory Cytotoxic T Lymphocyte Formation.
Dapeng Wang,Huitian Diao,Adam J. Getzler,Walter Rogal,Megan A. Frederick,J. Justin Milner,Bingfei Yu,Shane Crotty,Ananda W. Goldrath,Matthew E. Pipkin +9 more
TL;DR: Runx3 governs chromatin accessibility during TCR stimulation and enforces the memory CTL developmental program during iterative infections.
Journal ArticleDOI
Low CD8 T-Cell Proliferative Potential and High Viral Load Limit the Effectiveness of Therapeutic Vaccination
TL;DR: It is demonstrated that therapeutic vaccination using a recombinant vaccinia virus expressing the LCMV GP33 CD8 T-cell epitope can be effective at accelerating viral control, and evidence is provided that a high viral load at the time of vaccination and the low proliferative potential of responding T cells are likely to limit the effectiveness of therapeutic vaccination.
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