Journal ArticleDOI
In vivo CD8+ T cell CRISPR screening reveals control by Fli1 in infection and cancer.
Zeyu Chen,Eri Arai,Omar Khan,Zhen Zhang,Shin Foong Ngiow,Yuan He,Hua Huang,Sasikanth Manne,Zhendong Cao,Amy E. Baxter,Zhangying Cai,Elizabeth Freilich,Mohammed Alkhatim A. Ali,Josephine R. Giles,Jennifer E. Wu,Allison R. Greenplate,Mohamed A. Hakeem,Qingzhou Chen,Makoto Kurachi,Kito Nzingha,Viktoriya Ekshyyan,Divij Mathew,Zhuoyu Wen,Nancy A. Speck,Alexis Battle,Shelley L. Berger,E. John Wherry,Junwei Shi +27 more
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TLDR
In this paper, the authors developed an in-vivo T-cell CRISPR screening platform and identified a key mechanism restraining TEFF biology through the ETS family TF, Fli1.About:
This article is published in Cell.The article was published on 2021-03-04. It has received 74 citations till now.read more
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High-content CRISPR screening
TL;DR: Bock et al. as mentioned in this paper described the basic and advanced concepts of CRISPR screening and its application as a flexible and reliable method for biological discovery, biomedical research and drug development, with a special emphasis on high-content methods that make it possible to obtain detailed biological insights directly as part of the screen.
Journal ArticleDOI
High-content CRISPR screening
Christopher J Bock,Paul Datlinger,Florence M. Chardon,Matthew A. Coelho,Matthew B. Dong,Keith A. Lawson,Tian Lu,Laetitia Maroc,Thomas M. Norman,Bicna Song,Geoff Stanley,Sidi Chen,Mathew J. Garnett,Wei Li,Jason Moffat,Lei S. Qi,Rebecca S. Shapiro,Jay Shendure,Jonathan S. Weissman,Xiao-Xi Zhuang +19 more
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A genome-scale screen for synthetic drivers of T cell proliferation
Mateusz Legut,Zoran Gajic,Maria Francesca Guarino,Zharko Daniloski,J. Rahman,Xinhe Xue,Congyi Lu,Lu Lu,Eleni P. Mimitou,Stephanie Hao,Teresa Davoli,Catherine Diefenbach,Peter Smibert,Neville E. Sanjana +13 more
TL;DR: In this paper , the authors identify positive regulators of T cell functions through overexpression of around 12,000 barcoded human open reading frames (ORFs), which increased the proliferation and activation of primary human CD4+ and CD8+ T cells and their secretion of key cytokines such as interleukin-2 and interferonγ.
Journal ArticleDOI
Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence
TL;DR: In this paper , the authors provided an atlas of the genetic regulators of T cell exhaustion and demonstrated that modulation of epigenetic state can improve T cell responses in cancer immunotherapy, which led to improved antitumor immunity.
Journal ArticleDOI
A genome-scale screen for synthetic drivers of T cell proliferation
Mateusz Legut,Zoran Gajic,Maria Francesca Guarino,Zharko Daniloski,J. Rahman,Xinhe Xue,Congyi Lu,Lu Lu,Eleni P. Mimitou,Stephanie Hao,Teresa Davoli,Catherine Diefenbach,Peter Smibert,Neville E. Sanjana +13 more
TL;DR: In this article , the authors identify positive regulators of T cell functions through overexpression of around 12,000 barcoded human open reading frames (ORFs), which increased the proliferation and activation of primary human CD4+ and CD8+ T cells and their secretion of key cytokines such as interleukin-2 and interferonγ.
References
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Journal ArticleDOI
A major chromatin regulator determines resistance of tumor cells to T cell–mediated killing
Deng Pan,Aya Kobayashi,Aya Kobayashi,Peng Jiang,Lucas Ferrari de Andrade,Rong En Tay,Adrienne M. Luoma,Daphne Tsoucas,Xintao Qiu,Klothilda Lim,Prakash Rao,Henry W. Long,Guo-Cheng Yuan,John G. Doench,Myles Brown,X. Shirley Liu,Kai W. Wucherpfennig +16 more
TL;DR: A genome-scale CRISPR-Cas9 screen was used to identify mechanisms of tumor cell resistance to killing by cytotoxic T cells, the central effectors of antitumor immunity.
Journal ArticleDOI
Chromatin states define tumour-specific T cell dysfunction and reprogramming
Mary Philip,Lauren Fairchild,Lauren Fairchild,Liping Sun,Ellen L. Horste,Steven Camara,Mojdeh Shakiba,Mojdeh Shakiba,Andrew Scott,Andrew Scott,Agnes Viale,Peter Lauer,Taha Merghoub,Taha Merghoub,Matthew D. Hellmann,Matthew D. Hellmann,Jedd D. Wolchok,Jedd D. Wolchok,Christina S. Leslie,Andrea Schietinger,Andrea Schietinger +20 more
TL;DR: It is shown that T cells in mouse tumours differentiate through two discrete chromatin states: a plastic dysfunctional state from which T cells can be rescued, and a fixed dysfunctional state in which the cells are resistant to reprogramming.
Journal ArticleDOI
Differentiation and persistence of memory CD8+ T cells depend on T cell factor 1
TL;DR: It is shown that TCF-1 deficiency limited proliferation of CD8(+) effector T cells and impaired their differentiation toward a central memory phenotype, and forced expression of Eomes partly protected TCf-1-deficient memory CD 8(+) T cells from time-dependent attrition.
Journal ArticleDOI
Co-expression of CD39 and CD103 identifies tumor-reactive CD8 T cells in human solid tumors
Thomas Duhen,Rebekka Duhen,Ryan Montler,Jake Moses,Tarsem Moudgil,Noel F C C de Miranda,Cheri P. Goodall,Tiffany C. Blair,Bernard A. Fox,Jason E. McDermott,Shu Ching Chang,Gary L. Grunkemeier,Rom Leidner,R.B. Bell,Andrew D. Weinberg +14 more
TL;DR: The authors show that CD39 and CD103 mark a subset of tumor-infiltrating CD8 T cells that are tumor-reactive and exhibit characteristics of exhausted or tissue-resident memory T cells, which may lead to future adoptive T-cell cancer therapies.
Journal ArticleDOI
The Transcription Factor NFAT Promotes Exhaustion of Activated CD8+ T Cells
Gustavo J. Martinez,Renata M. Pereira,Tarmo Äijö,Tarmo Äijö,Edward Y. Kim,Francesco Marangoni,Matthew E. Pipkin,Matthew E. Pipkin,Susan Togher,Vigo Heissmeyer,Yi Chen Zhang,Shane Crotty,Edward D. Lamperti,K. Mark Ansel,Thorsten R. Mempel,Harri Lähdesmäki,Patrick G. Hogan,Anjana Rao +17 more
TL;DR: It is demonstrated that the transcription factor NFAT controls the program of T cell exhaustion and promotes T cell anergy and exhaustion by binding at sites that do not require cooperation with AP-1.
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