Journal ArticleDOI
In vivo CD8+ T cell CRISPR screening reveals control by Fli1 in infection and cancer.
Zeyu Chen,Eri Arai,Omar Khan,Zhen Zhang,Shin Foong Ngiow,Yuan He,Hua Huang,Sasikanth Manne,Zhendong Cao,Amy E. Baxter,Zhangying Cai,Elizabeth Freilich,Mohammed Alkhatim A. Ali,Josephine R. Giles,Jennifer E. Wu,Allison R. Greenplate,Mohamed A. Hakeem,Qingzhou Chen,Makoto Kurachi,Kito Nzingha,Viktoriya Ekshyyan,Divij Mathew,Zhuoyu Wen,Nancy A. Speck,Alexis Battle,Shelley L. Berger,E. John Wherry,Junwei Shi +27 more
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TLDR
In this paper, the authors developed an in-vivo T-cell CRISPR screening platform and identified a key mechanism restraining TEFF biology through the ETS family TF, Fli1.About:
This article is published in Cell.The article was published on 2021-03-04. It has received 74 citations till now.read more
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High-content CRISPR screening
TL;DR: Bock et al. as mentioned in this paper described the basic and advanced concepts of CRISPR screening and its application as a flexible and reliable method for biological discovery, biomedical research and drug development, with a special emphasis on high-content methods that make it possible to obtain detailed biological insights directly as part of the screen.
Journal ArticleDOI
High-content CRISPR screening
Christopher J Bock,Paul Datlinger,Florence M. Chardon,Matthew A. Coelho,Matthew B. Dong,Keith A. Lawson,Tian Lu,Laetitia Maroc,Thomas M. Norman,Bicna Song,Geoff Stanley,Sidi Chen,Mathew J. Garnett,Wei Li,Jason Moffat,Lei S. Qi,Rebecca S. Shapiro,Jay Shendure,Jonathan S. Weissman,Xiao-Xi Zhuang +19 more
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A genome-scale screen for synthetic drivers of T cell proliferation
Mateusz Legut,Zoran Gajic,Maria Francesca Guarino,Zharko Daniloski,J. Rahman,Xinhe Xue,Congyi Lu,Lu Lu,Eleni P. Mimitou,Stephanie Hao,Teresa Davoli,Catherine Diefenbach,Peter Smibert,Neville E. Sanjana +13 more
TL;DR: In this paper , the authors identify positive regulators of T cell functions through overexpression of around 12,000 barcoded human open reading frames (ORFs), which increased the proliferation and activation of primary human CD4+ and CD8+ T cells and their secretion of key cytokines such as interleukin-2 and interferonγ.
Journal ArticleDOI
Genome-wide CRISPR screens of T cell exhaustion identify chromatin remodeling factors that limit T cell persistence
TL;DR: In this paper , the authors provided an atlas of the genetic regulators of T cell exhaustion and demonstrated that modulation of epigenetic state can improve T cell responses in cancer immunotherapy, which led to improved antitumor immunity.
Journal ArticleDOI
A genome-scale screen for synthetic drivers of T cell proliferation
Mateusz Legut,Zoran Gajic,Maria Francesca Guarino,Zharko Daniloski,J. Rahman,Xinhe Xue,Congyi Lu,Lu Lu,Eleni P. Mimitou,Stephanie Hao,Teresa Davoli,Catherine Diefenbach,Peter Smibert,Neville E. Sanjana +13 more
TL;DR: In this article , the authors identify positive regulators of T cell functions through overexpression of around 12,000 barcoded human open reading frames (ORFs), which increased the proliferation and activation of primary human CD4+ and CD8+ T cells and their secretion of key cytokines such as interleukin-2 and interferonγ.
References
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Journal ArticleDOI
The Chemokine Receptor CX3CR1 Defines Three Antigen-Experienced CD8 T Cell Subsets with Distinct Roles in Immune Surveillance and Homeostasis
Carmen Gerlach,E. Ashley Moseman,Scott Loughhead,David Alvarez,Anthonie J. Zwijnenburg,Lisette Waanders,Rohit Garg,Juan Carlos de la Torre,Ulrich H. von Andrian,Ulrich H. von Andrian +9 more
TL;DR: A subset of CX3CR1int Tmem cells present unique phenotypic, homeostatic, and migratory properties, and it is proposed that tpm cells are chiefly responsible for the global surveillance of non-lymphoid tissues.
Journal ArticleDOI
CD8+ T cell contraction is controlled by early inflammation
TL;DR: Antibiotic treatment before Listeria monocytogenes infection induced numbers of protective memory CD8+ T cells similar to those in control infected mice, by a pathway without contraction, and the absence of contraction correlated with decreased early inflammation and interferon-γ production.
Journal ArticleDOI
The TCF1-Bcl6 axis counteracts type I interferon to repress exhaustion and maintain T cell stemness.
Tuoqi Wu,Yun Ji,E. Ashley Moseman,Haifeng C. Xu,Monica Manglani,Martha Kirby,Stacie M. Anderson,Robin Handon,Elizabeth Kenyon,Abdel G. Elkahloun,Weiwei Wu,Philipp A. Lang,Luca Gattinoni,Dorian B. McGavern,Pamela L. Schwartzberg +14 more
TL;DR: Insight is provided into the requirements for persistence of T cell immune responses in the face of exhaustion and mechanisms by which effective T cell–mediated immunity may be enhanced during chronic infections and cancer are suggested.
Journal ArticleDOI
CD4+ T cell help is required for the formation of a cytolytic CD8+ T cell subset that protects against chronic infection and cancer
Ryan Zander,David M. Schauder,Gang Xin,Christine Nguyen,Xiaopeng Wu,Allan J. Zajac,Weiguo Cui,Weiguo Cui +7 more
TL;DR: The findings uncovered the formation of a CX3CR1-expressing CD8+ T cell subset that exhibited potent cytolytic function and was required for viral control and have implications toward optimizing the generation of protective CD8- T cells in immunotherapy.
Journal ArticleDOI
TCF-1-Centered Transcriptional Network Drives an Effector Versus Exhausted CD8 T Cell-Fate Decision
Zeyu Chen,Zhicheng Ji,Shin Foong Ngiow,Sasikanth Manne,Zhangying Cai,Alexander C. Huang,John L. Johnson,Ryan P. Staupe,Bertram Bengsch,Caiyue Xu,Sixiang Yu,Makoto Kurachi,Ramin S. Herati,Laura A. Vella,Laura A. Vella,Amy E. Baxter,Jennifer E. Wu,Omar Khan,Jean Christophe Beltra,Josephine R. Giles,Erietta Stelekati,Laura M. McLane,Chi Wai Lau,Xiaolu Yang,Shelley L. Berger,Golnaz Vahedi,Hongkai Ji,E. John Wherry +27 more
TL;DR: A role for TCF-1 in early-fate-bifurcation-driving Tex precursor cells is defined and PD-1 is identified as a protector of this early TCf-1 subset.
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