Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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Current practices on genetic testing in ovarian cancer.
TL;DR: Which ovarian cancer patients should be referred for genetic counseling and how to perform genetic testing are discussed, and which patients with BRCA1/2 PVs or HR deficiency maintenance treatment with poly(ADP-ribose) polymerase (PARP) inhibitors improves the progression-free survival.
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Bağışıklık Yanıtından Genom Tasarımına; CRISPR-Cas9 Sistemi
TL;DR: In this review, CRISPR-Cas will be handled as an immune system regulator and immune system response steps will be defined in prokaryotic organisms, andCRISPR applications in cancer and therapeutic efficiency of genome editing technology will be evaluated.
Book ChapterDOI
Carcinogenesis: Mechanisms and Evaluation
TL;DR: In this article , the basic mechanisms of carcinogenesis, how these carcinogens are identified using in vitro and in vivo models, and how new and emerging technologies help accelerate drug development and the identification of carcinogenic compounds.
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Factors That Affect the Formation of Chromosomal Translocations in Cells
Reynand Jay C. Canoy,Anna A. Shmakova,Anna Karpukhina,M. V. Shepelev,Diego Germini,Yegor S. Vassetzky +5 more
TL;DR: Several factors may affect formation of chromosomal translocations, including chromatin motion to the potential sources of DSBs in the cell and how different cellular factors contribute to their formation.
Journal ArticleDOI
The Post-Synaptic Function of Brca2.
TL;DR: It is found that RAD51 in stoichiometric excess over ssDNA can cause D-loop disassembly in vitro and it is shown that this RAD51 activity is countered by BRCA2, demonstrating that BRC a2 may have a previously unexpected activity: regulation of HR at a post-synaptic stage by modulating RAD51-mediated D- loop dissociation.
References
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
Efficient genome editing in zebrafish using a CRISPR-Cas system
Woong Y. Hwang,Yanfang Fu,Deepak Reyon,Morgan L. Maeder,Shengdar Q. Tsai,Jeffry D. Sander,Randall T. Peterson,Randall T. Peterson,Jing-Ruey J. Yeh,J. Keith Joung +9 more
TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.