Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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New Human Chromosomal Sites with “Safe Harbor” Potential for Targeted Transgene Insertion
Stefan Pellenz,Michael Phelps,Weiliang Tang,Blake T. Hovde,Ryan B. Sinit,Wenqing Fu,Hui Li,Eleanor Y. Chen,Raymond J. Monnat +8 more
TL;DR: The most extensively characterized site on chromosome 4 (SHS231) was further characterized by targeted homology-dependent and -independent transgene insertion and expression in different human cell lines, and has the potential to serve as additional human SHS to enable basic and clinical gene editing and genome-engineering applications.
Journal ArticleDOI
Autocyclized and oxidized forms of SCR7 induce cancer cell death by inhibiting nonhomologous DNA end joining in a Ligase IV dependent manner
Supriya V. Vartak,Hassan A. Swarup,Vidya Gopalakrishnan,Vindya K. Gopinatha,Virginie Ropars,Mridula Nambiar,Franklin John,Sharath Kumar S. Kothanahally,Rupa Kumari,Nitu Kumari,Ujjayinee Ray,Gudapureddy Radha,Depina Dinesh,Monica Pandey,Hanumappa Ananda,Hanumappa Ananda,Subhas S. Karki,Mrinal Srivastava,Jean-Baptiste Charbonnier,Bibha Choudhary,Kempegowda Mantelingu,Sathees C. Raghavan +21 more
TL;DR: It is reported that cyclized and oxidized forms of SCR7 can inhibit NHEJ in a Ligase IV‐dependent manner, althoughSCR7‐pyrazine is less specific to Lig enzyme IV inside the cell, and both forms can potentiate the effect of radiation.
Journal ArticleDOI
Bromodomain proteins: repairing DNA damage within chromatin.
TL;DR: The involvement of acetylation signalling in the DDR is explored, focusing on the involvement of BRD proteins in promoting chromatin remodelling to repair DNA double-strand breaks.
Journal ArticleDOI
Genome-wide mapping of sister chromatid exchange events in single yeast cells using Strand-seq
Clémence Claussin,David Porubský,Diana C.J. Spierings,Nancy Halsema,Stefan Rentas,Victor Guryev,Peter M. Lansdorp,Peter M. Lansdorp,Peter M. Lansdorp,Michael Chang +9 more
TL;DR: It is provided the first quantifiable evidence that most spontaneous SCE events in wild-type cells are not due to the repair of DNA double-strand breaks, which is an indicator of elevated recombination activity and of genome instability, which are a hallmark of cancer.
Journal ArticleDOI
A Distinct Class of Genome Rearrangements Driven by Heterologous Recombination.
Ana María León-Ortiz,Stephanie Panier,Grzegorz Sarek,Jean-Baptiste Vannier,Harshil Patel,Peter J. Campbell,Simon J. Boulton +6 more
TL;DR: It is demonstrated that recombination is possible between heterologous sequences and that it is a source of chromosomal alterations in mitotic and meiotic cells and established Ht-REC as an unappreciated source of genome instability that underpins a novel class of complex genome rearrangements that likely arise during replication stress.
References
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Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
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RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
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Efficient genome editing in zebrafish using a CRISPR-Cas system
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TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.