Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Andrew V. Anzalone,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,David R. Liu,David R. Liu,David R. Liu +6 more
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CRISPR-Cas guides the future of genetic engineering.
TL;DR: The basic mechanisms that set the CRISPR-Cas toolkit apart from other programmable gene-editing technologies are described, highlighting the diverse and naturally evolved systems now functionalized as biotechnologies.
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Homologous Recombination and Human Health: The Roles of BRCA1, BRCA2, and Associated Proteins
TL;DR: This review summarizes recent findings on BRCA1, BRCa2, and associated proteins involved in human disease with an emphasis on their molecular roles and interactions.
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Recombination, Pairing, and Synapsis of Homologs during Meiosis
Denise Zickler,Nancy Kleckner +1 more
TL;DR: This review provides an overview of recombination-mediated processes in physical and functional linkage with meiotic axial chromosome structure, with interplay in both directions, before, during, and after formation and dissolution of the synaptonemal complex.
References
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Rad52 inactivation is synthetically lethal with BRCA2 deficiency
Zhihui Feng,Shaun P. Scott,Wendy Bussen,Girdhar G. Sharma,Gongshe Guo,Tej K. Pandita,Simon N. Powell +6 more
TL;DR: It is shown that loss of Rad52 function is synthetically lethal with breast cancer 2, early onset (BRCA 2) deficiency, whereas there was no impact on cell growth and viability in BRCA2-complemented cells.
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Homologous recombination in plant cells is enhanced by in vivo induction of double strand breaks into DNA by a site-specific endonuclease
TL;DR: The results imply that in vivo induction of transient breaks at specific sites in the plant genome could allow foreign DNA to be targeted to these sites via homologous recombination.
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Mechanisms that regulate localization of a DNA double-strand break to the nuclear periphery
TL;DR: It is proposed that sequestration of unrepaired or slowly repaired DSBs to the nuclear periphery reflects a competition between alternative repair pathways.
Journal ArticleDOI
Genetic predisposition to breast cancer: past, present, and future.
Clare Turnbull,Nazneen Rahman +1 more
TL;DR: The known genetic predisposition factors are described, the methods by which they were identified are expound on, and how further technological and intellectual advances may assist in identifying the remaining genetic factors underlying breast cancer susceptibility are considered.
Journal ArticleDOI
53BP1 facilitates long-range DNA end-joining during V(D)J recombination
Simone Difilippantonio,Eric J. Gapud,Nancy Wong,Ching-Yu Huang,Grace K. Mahowald,Hua Tang Chen,Michael J. Kruhlak,Elsa Callen,Ferenc Livak,Michel C. Nussenzweig,Barry P. Sleckman,André Nussenzweig +11 more
TL;DR: A previously unrecognized defect in the joining phase of V(D)J recombination in 53BP1-deficient lymphocytes is reported that is distinct from that found in classical non-homologous-end-joining-, H2ax-, Mdc1- and Atm- deficient mice, suggesting a more general role for 53BP 1 in maintaining genomic stability during long-range joining of DNA breaks.