Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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References
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Journal ArticleDOI
Changes in chromatin structure and mobility in living cells at sites of DNA double-strand breaks
Michael J. Kruhlak,Arkady Celeste,Graham Dellaire,Oscar Fernandez-Capetillo,Waltraud G. Müller,James G. McNally,David P. Bazett-Jones,André Nussenzweig +7 more
TL;DR: It is proposed that localized adenosine triphosphate–dependent decondensation of chromatin at DSBs establishes an accessible subnuclear environment that facilitates DNA damage signaling and repair.
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Mammalian SUMO E3-ligases PIAS1 and PIAS4 promote responses to DNA double-strand breaks
Yaron Galanty,Rimma Belotserkovskaya,Julia Coates,Sophie E. Polo,Kyle M. Miller,Stephen P. Jackson +5 more
TL;DR: It is established that PIAS1 and PIAS4 are required for effective ubiquitin-adduct formation mediated by RNF8, RNF168 and BRCA1 at sites of DNA damage and revealed how protein recruitment to DSB sites is controlled by coordinated SUMOylation and ubiquitylation.
Journal ArticleDOI
Double-strand breaks at an initiation site for meiotic gene conversion
TL;DR: It is shown that a double-strand break appears at the ARG4 recombination initiation site at the time of recombination, and that the broken DNA molecules end in long single-stranded tails.
Journal ArticleDOI
Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA
TL;DR: The rad51 homologues fail to complement the DNA repair defect of rad51 mutants of S. cerevisiae, and the 339 amino acid proteins predicted for the two mammalian genes are almost identical and are highly homologous (83%) with the yeast proteins.
Journal ArticleDOI
Double-Strand Breaks in Heterochromatin Move Outside of a Dynamic HP1a Domain to Complete Recombinational Repair
Irene Chiolo,Aki Minoda,Serafin U Colmenares,Aris Polyzos,Sylvain V. Costes,Gary H. Karpen,Gary H. Karpen +6 more
TL;DR: It is proposed that the spatial and temporal control of DSB repair in heterochromatin safeguards genome stability by preventing aberrant exchanges between repeats.