Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
Reads0
Chats0
TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
Citations
More filters
Journal ArticleDOI
Relationship between thyroid tumor radiosensitivity and nuclear localization of DNA-dependent protein kinase catalytic subunit
Makoto Ihara,Kazuko Shichijo,Kiyoto Ashizawa,Katsuya Matsuda,Ryota Otsubo,Ichiro Horie,Masahiro Nakashima,Takashi Kudo +7 more
TL;DR: Immunofluorescent staining showed that native DNA-PKcs was localized largely in the cytoplasm and only rarely localized in the nuclei of radiation-resistant thyroid cancer cells, whereas in radiation-sensitive cancer cells a 175-kDa cleaved C-terminal fragment of DNA- PKCS was localized mainly inside the nuclea.
Posted ContentDOI
Inhibiting POLQ-mediated alternative NHEJ enhances CRISPR/Cas9 mediated precise genome editing in CHO cells
Chuanjie Wang,Ming Wang,Mengmeng Zhang,Yao Wang,Xinying Li,Chenghua Liu,Rongrong Fan,Yuanqiang Zheng,Beifen Shen,Zhaolin Sun,Jing Wang,Jiannan Feng +11 more
TL;DR: In this paper , the DNA polymerase θ (POLθ), which is essential for Alt-EJ, was shown to significantly increase the knock-in efficiency in CHO cells via eGFP reporter system and does not affect the normal growth and proliferation of cells.
Posted ContentDOI
Centriolar subdistal appendages promote double strand break repair through homologous recombination
Guillermo Rodríguez-Real,Andrés Domínguez-Calvo,Rosario Prados-Carvajal,Aleix Bayona-Feliu,Fernando R. Balestra,Pablo Huertas +5 more
TL;DR: It is shown that centrosomes presence is required to fully activate recombination, specifically to completely license its initial step, the so-called DNA end resection, and loss of centrosome upregulates the non-homologous end-joining repair pathway.
Journal ArticleDOI
MND1 enables homologous recombination in somatic cells primarily outside the context of replication
Lisa Koob,Anoek Friskes,Louise van Bergen,Femke M. Feringa,Bram Van den Broek,Emma S. Koeleman,Vincent A. Blomen,Thijn R. Brummelkamp,Lenno Krenning,René H. Medema +9 more
TL;DR: In this article , the authors demonstrate that the meiotic recombination co-factor MND1 facilitates the repair of DSBs in somatic cells, where it stimulates DNA repair through homologous recombination.
Posted ContentDOI
Directed-evolution mutations of adenine base editor ABE8e improve its DNA-binding affinity and protein stability
TL;DR: Zhang et al. as discussed by the authors combined molecular dynamics simulations and experimental measurements to elucidate the molecular origin of the activity enhancement by these mutations, and showed that these mutations increase the positive charge density in the DNA-binding region, thereby enhancing the electrostatic attraction with DNA.
References
More filters
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
Efficient genome editing in zebrafish using a CRISPR-Cas system
Woong Y. Hwang,Yanfang Fu,Deepak Reyon,Morgan L. Maeder,Shengdar Q. Tsai,Jeffry D. Sander,Randall T. Peterson,Randall T. Peterson,Jing-Ruey J. Yeh,J. Keith Joung +9 more
TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.