Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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Andrew V. Anzalone,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,David R. Liu,David R. Liu,David R. Liu +6 more
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Recombination, Pairing, and Synapsis of Homologs during Meiosis
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References
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Journal ArticleDOI
A Switch from High-Fidelity to Error-Prone DNA Double-Strand Break Repair Underlies Stress-Induced Mutation
TL;DR: This work provides evidence that the molecular basis for stress-induced mutagenesis in an E. coli model is error-prone DNA double-strand break repair (DSBR), and reveals an RpoS-controlled switch from high-fidelity to mutagenic DSBR under stress.
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An essential role for CtIP in chromosomal translocation formation through an alternative end-joining pathway
Yu Zhang,Maria Jasin +1 more
TL;DR: It is shown that depletion of CtIP, a DNA end-resection factor, results in a substantial decrease in chromosomal translocation frequency in mouse cells, and it is directly demonstrated that CtIP-mediated alt-NHEJ has a primary role in translocation formation.
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Nuclear dynamics of RAD52 group homologous recombination proteins in response to DNA damage.
Jeroen Essers,Adriaan B. Houtsmuller,Lieneke R. van Veelen,Coen Paulusma,Alex L. Nigg,Albert Pastink,Wim Vermeulen,Jan H.J. Hoeijmakers,Roland Kanaar +8 more
TL;DR: This work shows that upon treatment of mammalian cells with ionizing radiation, RAD52 group proteins accumulate into foci at sites of DNA damage induction, and shows that these foci are dynamic structures of which Rad51 is a stably associated core component, whereas Rad52 and Rad54 rapidly and reversibly interact with the structure.
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Recognition and cleavage site of the intron-encoded omega transposase.
TL;DR: The optional group I intron of the mitochondrial 21S rRNA gene of Saccharomyces cerevisiae contains a 235-codon-long open reading frame the translation product of which (the omega transposase) catalyzes the formation of a double-strand break within the intron-minus (omega-) copies of the same gene.
Journal ArticleDOI
BRCA1 functions independently of homologous recombination in DNA interstrand crosslink repair.
Samuel F. Bunting,Elsa Callen,Marina L. Kozak,Jung Min Kim,Nancy Wong,Andrés J. López-Contreras,Thomas Ludwig,Richard Baer,Robert B. Faryabi,Amy Malhowski,Hua-Tang Chen,Oscar Fernandez-Capetillo,Alan D. D'Andrea,André Nussenzweig +13 more
TL;DR: It is reported that deletion of the DNA damage response factor 53BP1 overcomes embryonic lethality in Brca1-nullizygous mice and rescues HR deficiency, as measured by hypersensitivity to polyADP-ribose polymerase (PARP) inhibition.