Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors
Andrew V. Anzalone,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,David R. Liu,David R. Liu,David R. Liu +6 more
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TL;DR: The basic mechanisms that set the CRISPR-Cas toolkit apart from other programmable gene-editing technologies are described, highlighting the diverse and naturally evolved systems now functionalized as biotechnologies.
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Homologous Recombination and Human Health: The Roles of BRCA1, BRCA2, and Associated Proteins
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Recombination, Pairing, and Synapsis of Homologs during Meiosis
Denise Zickler,Nancy Kleckner +1 more
TL;DR: This review provides an overview of recombination-mediated processes in physical and functional linkage with meiotic axial chromosome structure, with interplay in both directions, before, during, and after formation and dissolution of the synaptonemal complex.
References
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Journal ArticleDOI
DNA Breaks Promote Genomic Instability by Impeding Proper Chromosome Segregation
Julia A. Kaye,Justine A. Melo,Stephanie K. Cheung,Moreshwar B. Vaze,James E. Haber,David P. Toczyski +5 more
TL;DR: Two related phenomena are suggested: an intrachromosomal association that holds the halves of a single broken sister chromatid together in metaphase and an interchromosomal force that tethers broken sister Chromatids to each other and promotes their missegregation.
Journal ArticleDOI
Targeted gene addition to a predetermined site in the human genome using a ZFN-based nicking enzyme
Jianbin Wang,Geoffrey Friedman,Yannick Doyon,Nathaniel Wang,Carrie Jiaxin Li,Jeffrey C. Miller,Kevin Hua,Jenny Jiacheng Yan,Joshua E. Babiarz,Philip D. Gregory,Michael C. Holmes +10 more
TL;DR: It is reported that ZFNs can be engineered to induce a site-specific DNA single-strand break (SSB) or nick and the potential for a SSB to direct repair pathway choice may prove advantageous for certain therapeutic applications such as the targeted correction of human disease-causing mutations.
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Mus81 and Yen1 Promote Reciprocal Exchange during Mitotic Recombination to Maintain Genome Integrity in Budding Yeast
TL;DR: Using an assay to detect unselected products of mitotic recombination, a significant decrease in crossovers is found in the Saccharomyces cerevisiae mus81Δ mutant, and Yen1 serves a backup function responsible for resolving intermediates in mus 81Δ mutants, or when conversion tracts are short.
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Tying synaptonemal complex initiation to the formation and programmed repair of DNA double-strand breaks
TL;DR: These results strongly correlate SC formation with Spo11 catalytic activity per se and strongly implicates crossover-designated recombination intermediates as the sites of SC initiation.
Journal ArticleDOI
DNA damage checkpoint and repair centers
Michael Lisby,Rodney Rothstein +1 more
TL;DR: In eukaryotes, recombinational repair is choreographed by multiprotein complexes that are organized into focal assemblies capable of simultaneously repairing multiple DNA lesions.