Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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Systematic analysis of long intergenic non-coding RNAs in C. elegans germline uncovers roles in somatic growth.
Hasan Ishtayeh,Hanna Achache,Eitan Kroizer,Yisrael Rappaport,Eyal Itskovits,Hila Gingold,Corinne Best,Oded Rechavi,Yonatan B Tzur +8 more
TL;DR: Analyzing the functions of lincRNAs in the genome of the nematode Caenorhabditis elegans showed that the somatic role stems from linc-4 expression in germline cells, which suggests that some l incRNAs, like some small non-coding RNAs, are required for germ-soma interactions.
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Chrysin impairs genomic stability by suppressing DNA double-strand break repair in breast cancer cells
Anke Geng,Shiquan Xu,Yunxia Yao,Zhen Qian,Xiyue Wang,Jiahui Sun,Jingyuan Zhang,Fangfang Shi,Zhixi Chen,Weina Zhang,Zhiyong Mao,Wen Wen Lu,Ying Jiang +12 more
TL;DR: It is demonstrated that chrysin impairs genomic stability in MCF-7 and BT474 cells, inhibits cell survival and enhances the sensitivity of MCf-7 cells to chemotherapeutic drugs, and proposed that a combination of chrys in and chemotherapy has curative potential in breast cancers.
Journal ArticleDOI
Resistance to UV Irradiation Caused by Inactivation of nurA and herA Genes in Thermus thermophilus.
TL;DR: The results suggest that the NurA-HerA complex has an architecture similar to that of archaeal counterparts but that it impairs, rather than promotes, the repair of photoproducts and DNA cross-links in T. thermophilus cells.
Journal ArticleDOI
A Curative DNA Code for Hematopoietic Defects Novel Cell Therapies for Monogenic Diseases of the Blood and Immune System
TL;DR: The authors reviewed the latest technological advances in the CRISPR/Cas9 system alone and combined with engineered viruses as editing tools for human hematopoietic stem and progenitor cells (HSPCs).
Dissertation
Genetic susceptibility to myeloproliferative neoplasms and therapeutic efficacy
TL;DR: This chapter discusses the genesis of the myELOPROLIFERATIVE NEOPLASMS, and the role of the BCR-ABL V280G MUTATION POTENTIAL ROLE in IMATINIB RESISTANCE: first case report.
References
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
Efficient genome editing in zebrafish using a CRISPR-Cas system
Woong Y. Hwang,Yanfang Fu,Deepak Reyon,Morgan L. Maeder,Shengdar Q. Tsai,Jeffry D. Sander,Randall T. Peterson,Randall T. Peterson,Jing-Ruey J. Yeh,J. Keith Joung +9 more
TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.