Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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Andrew V. Anzalone,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,David R. Liu,David R. Liu,David R. Liu +6 more
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Recombination, Pairing, and Synapsis of Homologs during Meiosis
Denise Zickler,Nancy Kleckner +1 more
TL;DR: This review provides an overview of recombination-mediated processes in physical and functional linkage with meiotic axial chromosome structure, with interplay in both directions, before, during, and after formation and dissolution of the synaptonemal complex.
References
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Numerical constraints and feedback control of double-strand breaks in mouse meiosis
Liisa Kauppi,Marco Barchi,Marco Barchi,Julian Lange,Frédéric Baudat,Frédéric Baudat,Maria Jasin,Scott Keeney +7 more
TL;DR: It is concluded that homolog pairing requirements dictate DSB set points during meiosis, and karyotype is a key factor: Smaller autosomes and heteromorphic sex chromosomes become weak links when DSBs are reduced below a critical threshold.
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Histone methyltransferase Dot1 and Rad9 inhibit single‐stranded DNA accumulation at DSBs and uncapped telomeres
Federico Lazzaro,Vasileia Sapountzi,Granata M,Achille Pellicioli,Moreshwar B. Vaze,James E. Haber,Paolo Plevani,David Lydall,Marco Muzi-Falconi +8 more
TL;DR: In this paper, Rad9 binding to histone H3-K79 methylated histone methylation has been shown to inhibit resection at DSBs and uncapped telomeres.
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Precision genome engineering with programmable DNA-nicking enzymes
TL;DR: It is proposed that programmable nickases will be of broad utility in research, medicine, and biotechnology, enabling precision genome engineering in any cell or organism.
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Homologous integration in mammalian cells without target gene selection.
Maria Jasin,Paul Berg +1 more
TL;DR: The experimental design, in which a defective marker is activated following a homologous integration, may have general applications for gene targeting in mammalian cells.
Journal ArticleDOI
Formation of NHEJ-derived reciprocal chromosomal translocations does not require Ku70.
TL;DR: It is shown that translocations are recovered from the joining of RAG-generated double-strand breaks (DSBs) to an endonuclease-generated DSB on a second chromosome, providing evidence for the participation of non-RAG DSBs in some lymphoid translocations.