Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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The Helicase PIF1 Facilitates Resection over Sequences Prone to Forming G4 Structures
Sonia Jimeno,Sonia Jimeno,Rosa Camarillo,Rosa Camarillo,Fernando Mejías-Navarro,Fernando Mejías-Navarro,María Jesús Fernández-Ávila,Isabel Soria-Bretones,Isabel Soria-Bretones,Rosario Prados-Carvajal,Rosario Prados-Carvajal,Pablo Huertas,Pablo Huertas +12 more
TL;DR: Evidence is found that the human helicase PIF1 has a role in DNA resection, specifically for defined DNA regions, such as those prone to form G-quadruplexes.
Posted ContentDOI
Systematic gene tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization
Brock Roberts,Amanda Haupt,Andrew Tucker,Tanya Grancharova,Joy Arakaki,Margaret A. Fuqua,Angelique M. Nelson,Caroline Hookway,Susan A. Ludmann,Irina A. Mueller,Ruian Yang,Alan R. Horwitz,Susanne M. Rafelski,Ruwanthi N. Gunawardane +13 more
TL;DR: The CRISPR/Cas9 genome editing strategy to systematically tag endogenous proteins with fluorescent tags in human inducible pluripotent stem cells can serve as an initial resource for genome editing in cell biology and stem cell research.
Journal ArticleDOI
TPX2/Aurora kinase A signaling as a potential therapeutic target in genomically unstable cancer cells
Stephanie E van Gijn,Elles Wierenga,Nathalie van den Tempel,Yannick P Kok,Anne Margriet Heijink,Diana C.J. Spierings,Floris Foijer,Marcel A. T. M. van Vugt,Rudolf S N Fehrmann +8 more
TL;DR: The findings reveal that BRCA2-deficient cancer cells show enhanced sensitivity to inactivation of TPX2 or its partner Aurora-A, which points at an actionable dependency of genomically unstable cancers.
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A toolbox of stable integration vectors in the fission yeast Schizosaccharomyces pombe.
Aleksandar Vjestica,Magdalena Marek,Pedro Junior Nkosi,Laura Merlini,Gaowen Liu,Melvin Bérard,Ingrid Billault-Chaumartin,Sophie G. Martin +7 more
TL;DR: A new vector series is established to efficiently and stably introduce DNA sequences into the fission yeast genome, and a large set of ready-to-use, fluorescent probes are generated to mark organelles and cellular processes with a wide range of applications in fissions yeast research.
Journal ArticleDOI
Efficient CRISPR/Cas9-Mediated Versatile, Predictable, and Donor-Free Gene Knockout in Human Pluripotent Stem Cells.
Zhongliang Liu,Yi Hui,Lei Shi,Zhenyu Chen,Xiangjie Xu,Liankai Chi,Beibei Fan,Yujiang Fang,Yang Liu,Lin Ma,Yiran Wang,Lei Xiao,Quan-bin Zhang,Guohua Jin,Ling Liu,Xiaoqing Zhang +15 more
TL;DR: This work suggests that the paired-KO strategy is a simple and robust system for loss-of-function studies for both coding and non-coding genes in hPSCs.
References
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A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
Efficient genome editing in zebrafish using a CRISPR-Cas system
Woong Y. Hwang,Yanfang Fu,Deepak Reyon,Morgan L. Maeder,Shengdar Q. Tsai,Jeffry D. Sander,Randall T. Peterson,Randall T. Peterson,Jing-Ruey J. Yeh,J. Keith Joung +9 more
TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.