Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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Dual DNA-binding domains shape the interaction of Brh2 with DNA
Qingwen Zhou,William K. Holloman +1 more
TL;DR: While the two DNA-binding regions of Brh2 appear functionally redundant in certain aspects of DNA repair, they differ in fundamental properties, and likely contribute in different ways to repair processes involving or arising from stalled DNA replication forks.
Journal ArticleDOI
Roles of homologous recombination in response to ionizing radiation-induced DNA damage.
Jac A. Nickoloff,Neelam Sharma,Christopher P. Allen,Lynn Taylor,Sage J. Allen,Aruna S. Jaiswal,Robert Hromas +6 more
TL;DR: In this paper, the role of error-prone nonhomologous end-joining (NHEJ) and generally error-free homologous recombination (HR) in cellular responses to ionizing radiation was discussed.
Journal ArticleDOI
Fluorescent tagging of endogenous Heme oxygenase-1 in human induced pluripotent stem cells for high content imaging of oxidative stress in various differentiated lineages.
Kirsten E. Snijders,Anita Fehér,Zsuzsanna Táncos,István Bock,Annamária Téglási,Linda van den Berk,Marije Niemeijer,Peter Bouwman,Sylvia E. Le Dévédec,Martijn J Moné,Rob Van Rossom,Manoj Kumar,Anja Wilmes,Paul Jennings,Catherine M. Verfaillie,Julianna Kobolák,Bas ter Braak,András Dinnyés,Bob van de Water +18 more
TL;DR: In this paper, the authors used CRISPR/Cas9 technology to insert an enhanced green fluorescent protein (eGFP) at the C-terminal end of the endogenous HMOX1 gene.
Journal ArticleDOI
Jumping Ahead with Sleeping Beauty: Mechanistic Insights into Cut-and-Paste Transposition.
Matthias T Ochmann,Zoltán Ivics +1 more
TL;DR: In this paper, structural data on the Sleeping Beauty transposase have been obtained to enable a direct insight into the workings of this enzyme, enabling the development of novel variants of SB that offer advanced possibilities for genetic engineering due to their hyperactivity, integration deficiency or targeting capacity.
Journal ArticleDOI
Targeting DNA Damage Repair Mechanisms in Pancreas Cancer
Lukas Perkhofer,Talia Golan,Pieter-Jan Cuyle,Tamara Matysiak-Budnik,Jean-Luc Van Laethem,Teresa Macarulla,Estelle Cauchin,Alexander Kleger,Alica K Beutel,Johann Gout,Albrecht Stenzinger,Eric Van Cutsem,Joaquim Bellmunt,Pascal Hammel,Eileen M. O'Reilly,Thomas Seufferlein +15 more
TL;DR: In this article, an expert panel was invited by the European Society of Digestive Oncology (ESDO) to assess the current knowledge and significance of DDR as a target in PDAC treatment.
References
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Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
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Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
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RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
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Efficient genome editing in zebrafish using a CRISPR-Cas system
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TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.