Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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Andrew V. Anzalone,Luke W. Koblan,Luke W. Koblan,Luke W. Koblan,David R. Liu,David R. Liu,David R. Liu +6 more
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TL;DR: The basic mechanisms that set the CRISPR-Cas toolkit apart from other programmable gene-editing technologies are described, highlighting the diverse and naturally evolved systems now functionalized as biotechnologies.
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Homologous Recombination and Human Health: The Roles of BRCA1, BRCA2, and Associated Proteins
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Recombination, Pairing, and Synapsis of Homologs during Meiosis
Denise Zickler,Nancy Kleckner +1 more
TL;DR: This review provides an overview of recombination-mediated processes in physical and functional linkage with meiotic axial chromosome structure, with interplay in both directions, before, during, and after formation and dissolution of the synaptonemal complex.
References
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Journal ArticleDOI
The genetic control of sporulation in Saccharomyces. II. Dominance and complementation of mutants of meiosis and spore formation.
TL;DR: Comparison of the wild type and mutants by light microscopy after exposure to sporulation medium at the restrictive temperature and Giemsa staining has shown that mutant populations can not complete the meiotic nuclear divisions.
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Alternative Pathways for the Repair of RAG-Induced DNA Breaks
David M. Weinstock,Maria Jasin +1 more
TL;DR: It is determined that RAG-generated chromosomal breaks can be repaired by pathways other than NHEJ in mouse embryonic stem (ES) cells, although repair by these pathways occurs at a significantly lower frequency than N HEJ.
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Analysis of intrachromosomal homologous recombination in mammalian cell, using tandem repeat sequences.
Sarah Lambert,Yannick Saintigny,Fabien Delacôte,F Amiot,B Chaput,M Lecomte,Sylvie Huck,Pascale Bertrand,Bernard S. Lopez +8 more
TL;DR: The interest of using intrachromosomal tandem repeat sequences to measure HR in mammalian cells is presented and the differences with the ectopic plasmids recombination are discussed.
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Lab-on-Chip for fast 3D particle tracking in living cells.
Houssam Hajjoul,Houssam Hajjoul,Silvia Kocanova,Silvia Kocanova,Imen Lassadi,Imen Lassadi,Kerstin Bystricky,Kerstin Bystricky,Aurélien Bancaud,Aurélien Bancaud +9 more
TL;DR: A novel Lab-on-Chip technology for3D particle tracking in living cells based on V-shaped micro-mirrors, which are used to observe fluorescent specimens from multiple vantage points, providing simultaneous stereo-images that can be recombined for 3D reconstruction.
Journal ArticleDOI
Suppression of the Double-Strand-Break-Repair Defect of the Saccharomyces Cerevisiae Rad57 Mutant
TL;DR: The Rad51 paralogs Rad55 and Rad57 form a heterodimer required to mediate the formation and/or stabilization of the Rad51 filament that appears to have a unique function in spontaneous recombination that is not essential for DSB repair.