Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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References
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Journal ArticleDOI
RNF8 Ubiquitylates Histones at DNA Double-Strand Breaks and Promotes Assembly of Repair Proteins
Niels Mailand,Simon Bekker-Jensen,Helene Faustrup,Fredrik Melander,Jiri Bartek,Claudia Lukas,Jiri Lukas +6 more
TL;DR: It is suggested that MDC1-mediated and RNF8-executed histone ubiquitylation protects genome integrity by licensing the DSB-flanking chromatin to concentrate repair factors near the DNA lesions.
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MDC1 Directly Binds Phosphorylated Histone H2AX to Regulate Cellular Responses to DNA Double-Strand Breaks
Manuel Stucki,Julie A. Clapperton,Duaa H. Mohammad,Michael B. Yaffe,Stephen J. Smerdon,Stephen P. Jackson +5 more
TL;DR: It is shown that MDC1/NFBD1-gammaH2AX complex formation regulates H2AX phosphorylation and is required for normal radioresistance and efficient accumulation of DNA-damage-response proteins on damaged chromatin.
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The Bloom's syndrome helicase suppresses crossing over during homologous recombination
Leonard Wu,Ian D. Hickson +1 more
TL;DR: It is shown that mutations in BLM and hTOPO IIIα together effect the resolution of a recombination intermediate containing a double Holliday junction and prevents exchange of flanking sequences, which has wider implications for the understanding of the process of homologous recombination and the mechanisms that exist to prevent tumorigenesis.
Journal ArticleDOI
Insertion of DNA sequences into the human chromosomal beta-globin locus by homologous recombination.
TL;DR: A ‘rescuable’ plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmids integrated into the human β-globin locus.
Journal ArticleDOI
Dynamics of DNA damage response proteins at DNA breaks: a focus on protein modifications
TL;DR: How the development of various complementary methodologies has provided valuable insights into the spatiotemporal dynamics of DDR protein assembly/disassembly at sites of DNA strand breaks in eukaryotic cells is outlined.