Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
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TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
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Recombination, Pairing, and Synapsis of Homologs during Meiosis
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References
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Genetic dissection of vertebrate 53BP1: A major role in non-homologous end joining of DNA double strand breaks
Kyoko Nakamura,Wataru Sakai,Wataru Sakai,Takuo Kawamoto,Ronan T. Bree,Noel F. Lowndes,Shunichi Takeda,Yoshihito Taniguchi +7 more
TL;DR: It is concluded that the major role of 53BP1 in supporting survival of DT40 cells that have suffered DNA DSBs is in facilitating repair by NHEJ.
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Searching for synthetic lethality in cancer.
TL;DR: The biological concept of synthetic lethality has been successfully implemented to identify new therapeutic approaches and targets in models from yeast through to human cells and how recent technical advances combined with an increased understanding of cellular networks may facilitate therapeutic advances in the future are described.
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Evolutionary conservation of meiotic DSB proteins: more than just Spo11.
TL;DR: A phylogenomic approach was used to identify two meiotic-specific proteins across multiple clades, and it was confirmed that one of these, MEI4, is a functional ortholog in mouse.
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High frequency of homologous recombination in mammalian cells between endogenous and introduced SV40 genomes
TL;DR: The highest levels of wild-type virus were produced from transfections of molecules that contained a double-strand break at positions of uninterrupted homology with the chromosomal template, indicating recombination between introduced and genomic DNA.
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Limiting the Persistence of a Chromosome Break Diminishes Its Mutagenic Potential
Nicole Bennardo,Amanda Gunn,Amanda Gunn,Anita Cheng,Paul Hasty,Jeremy M. Stark,Jeremy M. Stark +6 more
TL;DR: Limiting the persistence of a DSB causes a reduction in the frequency of repair pathways that lead to significant genetic loss, and individual genetic factors play distinct roles during repair of non-cohesive DSB ends that are generated via co-expression of I-SceI with Trex2.