Repair of strand breaks by homologous recombination.
Maria Jasin,Rodney Rothstein +1 more
Reads0
Chats0
TLDR
The enzymology of the process is discussed, followed by studies on DSB repair in living cells, and a historical context for the current view of HR is provided and how DSBs are processed during HR as well as interactions with other D SB repair pathways are described.Abstract:
In this review, we discuss the repair of DNA double-strand breaks (DSBs) using a homologous DNA sequence (i.e., homologous recombination [HR]), focusing mainly on yeast and mammals. We provide a historical context for the current view of HR and describe how DSBs are processed during HR as well as interactions with other DSB repair pathways. We discuss the enzymology of the process, followed by studies on DSB repair in living cells. Whenever possible, we cite both original articles and reviews to aid the reader for further studies.read more
Citations
More filters
Patent
METHODS AND COMPOSITIONS FOR TARGETED GENETIC MODIFICATION USING PAIRED GUIDE RNAs
Andrew J. Murphy,David Frendewey,Ka-Man Venus Lai,Wojtek Auerbach,Jeffrey D. Lee,Mujica Alexander O,Gustavo Droguett,Sean Trzaska,Charleen Hunt,Anthony Gagliardi,Valenzuela David M,Vera Voronina,Macdonald Lynn,George D. Yancopoulos +13 more
TL;DR: In this paper, compositions and methods for modifying a genome within a cell that is heterozygous for an allele to become homozygous of that allele are provided. But the methods make use of Cas proteins and two or more guide RNAs that target different locations within the same genomic target locus.
Journal ArticleDOI
DNA Damage Repair in the Context of Plant Chromatin
TL;DR: This Update compares the knowledge of the role of chromatin and chromatin-modifying factors during DDR in plants with equivalent systems in yeast and humans to emphasize plant-specific elements and discuss possible implications.
Journal ArticleDOI
CRISPR technology: A decade of genome editing is only the beginning
Joy Y. Wang,Jennifer A. Doudna +1 more
TL;DR: Wang et al. as mentioned in this paper reviewed the origins and utility of CRISPR-based genome editing, the successes and current limitations of the technology, and where innovation and engineering are needed.
Journal ArticleDOI
CRISPR technologies for stem cell engineering and regenerative medicine.
TL;DR: In vitro stem cell fate manipulation and in vivo applications such as prevention of retinal and muscular degeneration, neural regeneration, bone regeneration, cartilage tissue engineering, and treatment of diseases in blood, skin and liver are focused on.
Journal ArticleDOI
ULK1 inhibition overcomes compromised antigen presentation and restores antitumor immunity in LKB1 mutant lung cancer
Jiehui Deng,Aatish Thennavan,Igor Dolgalev,Ting Chen,Jie Li,Antonio Marzio,John T. Poirier,David H. Peng,Mirna Bulatović,Subhadip Mukhopadhyay,Heather Silver,Eleni Papadopoulos,Val Pyon,Cassandra Thakurdin,Han Han,Fei Li,Shuai Li,Hailin Ding,Hai Hu,Yuanwang Pan,Vajira K. Weerasekara,Baishan Jiang,Eric S. Wang,Ian M. Ahearn,Mark R. Philips,Thales Papagiannakopoulos,Aristotelis Tsirigos,Eli Rothenberg,Justin F. Gainor,Gordon J. Freeman,Charles M. Rudin,Nathanael S. Gray,Peter S. Hammerman,Michele Pagano,John V. Heymach,Charles M. Perou,Nabeel Bardeesy,Kwok-Kin Wong +37 more
TL;DR: Wang et al. as mentioned in this paper found that LKB1 deficiency correlated with elevated tumor mutational burden (TMB) in nonsmokers and genetically engineered mouse models, despite the frequent association between high TMB and anti-PD-1 treatment efficacy.
References
More filters
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,Le Cong,F. Ann Ran,F. Ann Ran,David M. Cox,David M. Cox,Shuailiang Lin,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +15 more
TL;DR: The type II prokaryotic CRISPR (clustered regularly interspaced short palindromic repeats)/Cas adaptive immune system has been shown to facilitate RNA-guided site-specific DNA cleavage as discussed by the authors.
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. A. Ran,David Benjamin Turitz Cox,Shuailiang Lin,Robert P. J. Barretto,Naomi Habib,Patrick D. Hsu,Xuebing Wu,Wenyan Jiang,Luciano A. Marraffini,Feng Zhang +10 more
TL;DR: Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.
Journal ArticleDOI
RNA-Guided Human Genome Engineering via Cas9
Prashant Mali,Luhan Yang,Kevin M. Esvelt,John Aach,Marc Güell,James E. DiCarlo,Julie E. Norville,George M. Church,George M. Church +8 more
TL;DR: The type II bacterial CRISPR system is engineer to function with custom guide RNA (gRNA) in human cells to establish an RNA-guided editing tool for facile, robust, and multiplexable human genome engineering.
Journal ArticleDOI
Efficient genome editing in zebrafish using a CRISPR-Cas system
Woong Y. Hwang,Yanfang Fu,Deepak Reyon,Morgan L. Maeder,Shengdar Q. Tsai,Jeffry D. Sander,Randall T. Peterson,Randall T. Peterson,Jing-Ruey J. Yeh,J. Keith Joung +9 more
TL;DR: It is shown that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in zebrafish embryos with efficiencies similar to those obtained using zinc finger nucleases and transcription activator-like effector nucleases.