Journal ArticleDOI
The essentials of DNA methylation.
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This article is published in Cell.The article was published on 1992-07-10. It has received 928 citations till now. The article focuses on the topics: DNA methylation & Methylation.read more
Citations
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7-Deaza-2'-deoxyguanosine allows PCR and sequencing reactions from CpG islands.
TL;DR: The use of 7-deaza-2`-deoxyguanosine for PCR amplification of the human p16INK4A promoter and sequencing of HUMARA exon 1 PCR products significantly improves results, particularly when small amounts of poor quality DNA are available as starting material.
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A Human Modifier of Methylation for Class I HLA Genes (Memo-1) Maps to Chromosomal Bands 1p35–36.1
TL;DR: Methylation of HLA-C, -E and -A genes, which are encoded in a 1.4 Mb region on 6p21, is correlated in most cell lines, suggesting that 1p35-36.1 encodes a modifier of methylation for class I HLA genes, that is deleted in many neuroblastomas.
Journal ArticleDOI
Isolation and characterization of the rat gene for carbamoylphosphate synthetase-i
Maurice J.B. van den Hoff,Louis P. W. G. M. Van De Zande,Maria A. Dingemanse,Atze T. Das,Wil T. Labruyère,Antoon F.M. Moorman,R. Charles,Wouter H. Lamers +7 more
TL;DR: Transient expression assays revealed that the region surrounding the CCGG site at 6.3 kb functions as an enhancer in the vicinity of the basal promoter, suggesting that the hepatocyte-specific expression of CbmPS is related to tissue-specific differences in the sensitivity to cyclic AMP.
Journal ArticleDOI
DNA methylation status of the methylenetetrahydrofolate reductase gene promoter in peripheral blood of end-stage renal disease patients
TL;DR: Data generated from this study suggest the possible involvement of MTHFR promoter methylation in the pathogenesis of ESRD and support a new dimension of MthFR inactivation.
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Epigenetics in High‐Grade Astrocytomas
TL;DR: A new ubiquitous angiogenic factor in HGA is identified, a vascular endothelial growth factor‐D (VEGF‐D), and it is demonstrated that the activating protein‐1 (AP‐1) family of transcription factors play a potentially critical role in the progression of gliomas by elicit uncontrolled upregulation of VEGF‐D and other compounds essential for cancer cell proliferation, tumorigenesis, and infiltration.
References
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Journal ArticleDOI
Targeted mutation of the DNA methyltransferase gene results in embryonic lethality.
TL;DR: Results indicate that while a 3-fold reduction in levels of genomic m5C has no detectable effect on the viability or proliferation of ES cells in culture, a similar reduction of DNA methylation in embryos causes abnormal development and embryonic lethality.
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CpG-rich islands and the function of DNA methylation
TL;DR: It is likely that most vertebrate genes are associated with ‘HTF islands’—DNA sequences in which CpG is abundant and non-methylated; however, highly tissue-specific genes, though, usually lack islands.
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Purification, sequence, and cellular localization of a novel chromosomal protein that binds to Methylated DNA
Joe D. Lewis,Joe D. Lewis,Richard R. Meehan,Richard R. Meehan,William J. Henzel,Ingrid Maurer-Fogy,Peter Jeppesen,Franz Klein,Adrian Bird,Adrian Bird +9 more
TL;DR: This work reports the identification, purification, and cDNA cloning of a novel MeCP called MeCP2, which unlike MeCP1, the new protein is able to bind to DNA that contains a single methyl-CpG pair.
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Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
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DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein
Joan Boyes,Adrian Bird +1 more
TL;DR: Repression of transcription in vitro for four different promoters was shown to be an indirect effect and the mediator of repression had properties indistinguishable from those of a methyl-CpG binding protein (MeCP-1) that has been previously identified.