Journal ArticleDOI
The essentials of DNA methylation.
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This article is published in Cell.The article was published on 1992-07-10. It has received 928 citations till now. The article focuses on the topics: DNA methylation & Methylation.read more
Citations
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Journal ArticleDOI
Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands
TL;DR: The use of MSP is demonstrated to identify promoter region hypermethylation changes associated with transcriptional inactivation in four important tumor suppressor genes (p16, p15, E-cadherin and von Hippel-Lindau) in human cancer.
Book ChapterDOI
Alterations in dna methylation : a fundamental aspect of neoplasia
TL;DR: A model is proposed wherein tumor progression results from episodic clonal expansion of heterogeneous cell populations driven by continuous interaction between these methylation abnormalities and classic genetic changes.
Journal ArticleDOI
5' CpG island methylation is associated with transcriptional silencing of the tumour suppressor p16/CDKN2/MTS1 in human cancers.
Adrian Merlo,James G. Herman,Li Mao,Daniel J. Lee,Edward Gabrielson,Peter C. Burger,Stephen B. Baylin,David Sidransky +7 more
TL;DR: De novo methylation of the 5′ CpG island of p16 was found in approximately 20% of different primary neoplasms, but not in normal tissues, potentially representing a common pathway of tumour suppressor gene inactivation in human cancers.
Journal ArticleDOI
Incidence and functional consequences of hMLH1 promoter hypermethylation in colorectal carcinoma
James G. Herman,Asad Umar,Kornelia Polyak,Jeremy R. Graff,Nita Ahuja,Jean Pierre J. Issa,Sanford Markowitz,Sanford Markowitz,James K V Willson,Stanley R. Hamilton,Kenneth W. Kinzler,Michael F. Kane,Richard D. Kolodner,Bert Vogelstein,Thomas A. Kunkel,Stephen B. Baylin +15 more
TL;DR: The results suggest that microsatellite instability in sporadic colorectal cancer often results from epigenetic inactivation of hMLH1 in association with DNA methylation.
Journal ArticleDOI
Positional cloning of the mouse circadian clock gene.
David P. King,Yaliang Zhao,Ashvin M. Sangoram,Lisa D. Wilsbacher,Minoru Tanaka,Marina P. Antoch,Thomas D.L. Steeves,Martha Hotz Vitaterna,Jon M. Kornhauser,Jon M. Kornhauser,Phillip L. Lowrey,Fred W. Turek,Joseph S. Takahashi +12 more
TL;DR: CLOCK represents the second example of a PAS domain-containing clock protein (besides Drosophila PERIOD), which suggests that this motif may define an evolutionarily conserved feature of the circadian clock mechanism.
References
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Journal ArticleDOI
Methylation, mutation and cancer.
TL;DR: Comparison of the spectrum of mutations present in this gene in different human cancers allows for predictions to be made on the molecular mechanisms of tumorigenesis.
Journal ArticleDOI
Specific protection of methylated CpGs in mammalian nuclei
TL;DR: Quantitative analysis of limit digests showed that MspI and another methyl-CpG insensitive enzyme, Tth, have a strong bias against cutting methylated sites in these nuclei, suggesting that resistance to nucleases is mediated by factors that are bound specifically to methylated CpGs.
Journal ArticleDOI
CpG methylated minichromosomes become inaccessible for V(D)J recombination after undergoing replication.
Chih-Lin Hsieh,Michael R. Lieber +1 more
TL;DR: It is found that CpG methylation decreases the V(D)J recombination of these substrates more than 100‐fold, and this decrease correlates with a considerable increase in resistance to endonuclease digestion of the methylated minichromosome DNA.
Journal ArticleDOI
Dynamics of demethylation and activation of the α-actin gene in myoblasts
TL;DR: Transient transfection into L8 myoblasts has been used to study the rat alpha-actin gene promoter and demonstrated that demethylation of both strands at the critical CpG loci is essential to activate transcription.
Journal ArticleDOI
Muscle-specific activation of a methylated chimeric actin gene.
TL;DR: Transfected in vitro methylated alpha-actin (skeletal) constructs into fibroblasts and into a myogenic line reveal specific demethylations in the transfected molecules in myoblasts but not in fibro Blasts, and this pattern precisely mimics the methylation pattern found inMyoblasts in vivo.