Journal ArticleDOI
The essentials of DNA methylation.
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This article is published in Cell.The article was published on 1992-07-10. It has received 928 citations till now. The article focuses on the topics: DNA methylation & Methylation.read more
Citations
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DNA global hypomethylation in EBV-transformed interphase nuclei.
M Habib,F. Fares,Claire A. Bourgeois,C. Bella,J. Bernardino,Francisco Javier Hernandez-Blazquez,A. de Capoa,Alain Niveleau +7 more
TL;DR: An immunochemical technique was set up with the aim of demonstrating, in a population of mixed cells, the possibility of detecting the presence of individual nuclei containing hypomethylated DNA, on a cell-by-cell basis and opening the field for the analysis of fresh tumor samples by flow cytometry and microscopy.
Journal ArticleDOI
Chromatin and DNA sequences in defining promoters for transcription initiation
TL;DR: This review attempts to summarise these promoter features and discusses the available evidence pointing at their interactions in defining transcription initiation in developmental contexts.
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Transcriptional Regulation of the Human CYP1B1 Gene: EVIDENCE FOR INVOLVEMENT OF AN ARYL HYDROCARBON RECEPTOR RESPONSE ELEMENT IN CONSTITUTIVE EXPRESSION
TL;DR: The observed cell-specific differences in CYP1B1 constitutive expression are likely due to either 1) inaccessibility of the 5′-enhancer region in HepG2 cells to transcriptional activators due to a higher order chromatin structure that does not involve histone acetylation, or 2) the action of a repressor protein atcis-elements located outside of the –2296 to +25 region examined with the CYP 1B1 reporter constructs.
Journal ArticleDOI
Epigenetics in fertilization and preimplantation embryo development.
TL;DR: Some of the recent works which attempt to shed light into the importance of the dynamic epigenetic landscape observed during oocyte maturation and preimplantation embryo development in mammals are referenced.
Journal ArticleDOI
Targeted methylation of CMV and E1A viral promoters.
Chia-Chen Hsu,Hsin-Pai Li,Yu-Hung Hung,Yu-Wei Leu,Wu Hsiung Wu,Feng Sheng Wang,Kuan Der Lee,Pey-Jium Chang,Chi Sheng Wu,Yen Jung Lu,Tim H M Huang,Yu-Sun Chang,Shu-Huei Hsiao +12 more
TL;DR: TargetedDNA methylation is able to efficiently silence susceptible viral promoters and provides an alternative strategy to study the impact of loci-specific DNA methylation in viral gene expression.
References
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Journal ArticleDOI
Targeted mutation of the DNA methyltransferase gene results in embryonic lethality.
TL;DR: Results indicate that while a 3-fold reduction in levels of genomic m5C has no detectable effect on the viability or proliferation of ES cells in culture, a similar reduction of DNA methylation in embryos causes abnormal development and embryonic lethality.
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CpG-rich islands and the function of DNA methylation
TL;DR: It is likely that most vertebrate genes are associated with ‘HTF islands’—DNA sequences in which CpG is abundant and non-methylated; however, highly tissue-specific genes, though, usually lack islands.
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Purification, sequence, and cellular localization of a novel chromosomal protein that binds to Methylated DNA
Joe D. Lewis,Joe D. Lewis,Richard R. Meehan,Richard R. Meehan,William J. Henzel,Ingrid Maurer-Fogy,Peter Jeppesen,Franz Klein,Adrian Bird,Adrian Bird +9 more
TL;DR: This work reports the identification, purification, and cDNA cloning of a novel MeCP called MeCP2, which unlike MeCP1, the new protein is able to bind to DNA that contains a single methyl-CpG pair.
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Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
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DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein
Joan Boyes,Adrian Bird +1 more
TL;DR: Repression of transcription in vitro for four different promoters was shown to be an indirect effect and the mediator of repression had properties indistinguishable from those of a methyl-CpG binding protein (MeCP-1) that has been previously identified.