Journal ArticleDOI
The essentials of DNA methylation.
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This article is published in Cell.The article was published on 1992-07-10. It has received 928 citations till now. The article focuses on the topics: DNA methylation & Methylation.read more
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Oncogenic roles of DNA hypomethylation through the activation of cancer-germline genes
TL;DR: Together, this survey substantiates the concept that DNA hypomethylation promotes tumorigenesis via transcriptional activation of oncogenes and indicates that cancer-germline genes may represent valuable targets for the development of anti-cancer therapies with limited side effects.
Journal ArticleDOI
Epigenetic regulation of nervous system development by DNA methylation and histone deacetylation.
TL;DR: Evidence supporting a role for DNA methylation and histone deacetylation in nervous system development and mature function is reviewed, and a basis from which to understand how the clinical use of HDAC inhibitors may impact nervous system function is presented.
Patent
Cancer diagnostic method based upon DNA methylation differences
TL;DR: In this paper, a cancer diagnostic method based upon DNA methylation differences at specific CpG sites was disclosed, which provides for a bisulfite treatment of DNA, followed by methylation-sensitive single nucleotide primer extension (Ms-SNuPE), for determination of strand-specific methylation status at cytosine residues.
Journal ArticleDOI
Promoter hypomethylation of a novel cancer/testis antigen gene CAGE is correlated with its aberrant expression and is seen in premalignant stage of gastric carcinoma.
Bomsoo Cho,Hansoo Lee,Shin Wu Jeong,Yung-Jue Bang,Hyunjoo Lee,Kyu Sang Hwang,Hae-Yeong Kim,Yun Sil Lee,Gyeong Hoon Kang,Doo Il Jeoung +9 more
TL;DR: The results suggest that the methylation status of the CpG sites of Cage determines its expression, that the hypomethylation of CAGE precedes the development of gastric cancer and hepatocellular carcinoma, and that the high frequencies of hypometHylation, in various cancers would be valuable as a cancer diagnostic marker.
Journal ArticleDOI
Errors in the bisulfite conversion of DNA: modulating inappropriate- and failed-conversion frequencies
TL;DR: Molecule encoding was used to obtain validated, individual-molecule data on failed- and inappropriate-conversion frequencies for LowMT and HighMT treatments of both single-stranded and hairpin-linked oligonucleotides, finding that the HighMT treatment is preferable because it yields greater homogeneity among sites and among molecules in conversion rates, and thus yields more reliable data.
References
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Journal ArticleDOI
Targeted mutation of the DNA methyltransferase gene results in embryonic lethality.
TL;DR: Results indicate that while a 3-fold reduction in levels of genomic m5C has no detectable effect on the viability or proliferation of ES cells in culture, a similar reduction of DNA methylation in embryos causes abnormal development and embryonic lethality.
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CpG-rich islands and the function of DNA methylation
TL;DR: It is likely that most vertebrate genes are associated with ‘HTF islands’—DNA sequences in which CpG is abundant and non-methylated; however, highly tissue-specific genes, though, usually lack islands.
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Purification, sequence, and cellular localization of a novel chromosomal protein that binds to Methylated DNA
Joe D. Lewis,Joe D. Lewis,Richard R. Meehan,Richard R. Meehan,William J. Henzel,Ingrid Maurer-Fogy,Peter Jeppesen,Franz Klein,Adrian Bird,Adrian Bird +9 more
TL;DR: This work reports the identification, purification, and cDNA cloning of a novel MeCP called MeCP2, which unlike MeCP1, the new protein is able to bind to DNA that contains a single methyl-CpG pair.
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Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.
Shirley M. Taylor,Peter A. Jones +1 more
TL;DR: Three new mesenchymal phenotypes were expressed in cultures of Swiss 3T3 and C3H/10T1/2CL8 mouse cells treated with 5-azacytidine or 5-aza-2'-deoxycytidine, implying that cell division was obligatory for the expression of the new phenotypes.
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DNA methylation inhibits transcription indirectly via a methyl-CpG binding protein
Joan Boyes,Adrian Bird +1 more
TL;DR: Repression of transcription in vitro for four different promoters was shown to be an indirect effect and the mediator of repression had properties indistinguishable from those of a methyl-CpG binding protein (MeCP-1) that has been previously identified.