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The i5K initiative: Advancing arthropod genomics for knowledge, human health, agriculture, and the environment

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TLDR
An international effort to guide arthropod genomic efforts, from species prioritization to methodology and informatics is described, which aims to deliver sequences and analytical tools for each of theArthropod branches andEach of the species having beneficial and negative effects on humankind.
Abstract
Insects and their arthropod relatives including mites, spiders, and crustaceans play major roles in the world's terrestrial, aquatic, and marine ecosystems Arthropods compete with humans for food and transmit devastating diseases They also comprise the most diverse and successful branch of metazoan evolution, with millions of extant species Here, we describe an international effort to guide arthropod genomic efforts, from species prioritization to methodology and informatics The 5000 arthropod genomes initiative (i5K) community met formally in 2012 to discuss a roadmap for sequencing and analyzing 5000 high-priority arthropods and is continuing this effort via pilot projects, the development of standard operating procedures, and training of students and career scientists, With university, governmental, and industry support, the i5K Consortium aspires to deliver sequences and analytical tools for each of the arthropod branches and each of the species having beneficial and negative effects on humankind

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Journal ArticleDOI

Application of Genomics for Understanding Plant Virus-Insect Vector Interactions and Insect Vector Control.

TL;DR: These studies demonstrate the advantages of genomics and the potential complementary studies that rapidly advance the understanding of the biology of virus transmission by insect vector and offer additional opportunities to design novel genetic strategies to manage insect vectors and the viruses they transmit.
Journal ArticleDOI

Evaluation of reference genes for gene expression analysis by real-time quantitative PCR (qPCR) in three stingless bee species (Hymenoptera: Apidae: Meliponini).

TL;DR: To contribute to molecular studies on stingless bees, Frieseomelitta varia, Melipona quadrifasciata, and Scaptotrigona bipunctata species were used to test the expression stability of eight reference genes and the tpb-af gene showed the highest stability, thus being identified as suitable reference genes for the three stingless bee species and defined conditions.
Journal ArticleDOI

Universal fluorescence in situ hybridization (FISH) protocol for mapping repetitive DNAs in insects and other arthropods.

TL;DR: In this paper, a universal and affordable FISH protocol was proposed for the physical mapping of various sequences on chromosomes, such as multigene families, satellite DNAs, microsatellites, transposable elements, DOP-PCR products, and telomeric motif (TTAGG)n).
Journal ArticleDOI

A chromosome-scale draft sequence of the Canada fleabane genome.

TL;DR: As C. canadensis has a small genome that is syntenic with other Asteraceaes, a short life cycle, and is relatively easy to cross, it has the potential to become a model weed species and contribute to a paradigm shift in the way non-target site resistance is studied.
References
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Journal ArticleDOI

The genome sequence of Drosophila melanogaster

Mark Raymond Adams, +194 more
- 24 Mar 2000 - 
TL;DR: The nucleotide sequence of nearly all of the approximately 120-megabase euchromatic portion of the Drosophila genome is determined using a whole-genome shotgun sequencing strategy supported by extensive clone-based sequence and a high-quality bacterial artificial chromosome physical map.
Journal ArticleDOI

The genome of Tetranychus urticae reveals herbivorous pest adaptations

Miodrag Grbic, +60 more
- 24 Nov 2011 - 
TL;DR: The Tetranychus urticae genome is the smallest known arthropod genome as discussed by the authors, which represents the first complete chelicerate genome for a pest and has been annotated with genes associated with feeding on different hosts.
Journal ArticleDOI

Genome 10K: A Proposal to Obtain Whole-Genome Sequence for 10 000 Vertebrate Species

David Haussler, +69 more
- 01 Nov 2009 - 
TL;DR: A precipitous drop in costs and increase in sequencing efficiency is anticipated, with concomitant development of improved annotation technology, and it is proposed to create a collection of tissue and DNA specimens for 10,000 vertebrate species specifically designated for whole-genome sequencing in the very near future.
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