University of Kiel

About: University of Kiel is a education organization based out in Kiel, Germany. It is known for research contribution in the topics: Population & Crystal structure. The organization has 27816 authors who have published 57114 publications receiving 2061802 citations. The organization is also known as: Christian Albrechts University & Christian-Albrechts-Universität zu Kiel.

In vitro evaluation of bacterial leakage along the implant-abutment interface of different implant systems

Abstract: PURPOSE Microbial leakage and colonization between implants and their abutments may cause inflammatory reactions in the peri-implant tissues. This study evaluated microbial leakage at the implant-abutment interface with a new in vitro model. MATERIALS AND METHODS Bacterial leakage was tested during dynamic loading in a 2-axis chewing simulator. The authors theorized that dynamic loading would decrease the stability of the implant-abutment connections and thereby lead to bacterial penetration along the gap. Five different implant systems with 8 standard implant-abutment combinations for single molar crowns were tested. The internal aspects of the implants were inoculated with a bacterial suspension and connected to the superstructure with the recommended torque. The specimens were immersed in a nutrient solution and loaded with 1,200,000 cycles of 120 N in the chewing simulator. RESULTS Statistically significant differences (P < or = .05) between implant systems with respect to number of chewing cycles until bacterial penetration were found. DISCUSSION The degree of penetration in a specific implant system presumably is a multifactorial condition dependent on the precision of fit between the implant and the abutment, the degree of micromovement between the components, and the torque forces used to connect them. CONCLUSION It was concluded that the newly developed test model is a sensitive tool for the detection of differences between current implant systems with respect to their ability to prevent bacterial penetration at the implant-abutment interface under dynamic loading conditions.

Specification-oriented semantics for communicating processes

Abstract: A process P satisfies a specification S if every observation we can make of the behaviour of P is allowed by S. We use this idea of process correctness as a starting point for developing a specific form of denotational semantics for processes, called here specification — oriented semantics. This approach serves as a uniform framework for generating and relating a series of increasingly sophisticated denotational models for Communicating Processes. These models differ in the underlying structure of their observations which influences both the number of representable language operators and the induced notion of process correctness. Safety properties are treated by all models; the more sophisticated models also permit proofs of certain liveness properties. An important feature of the models is a special hiding operator which abstracts from internal process activity. This allows large processes to be composed hierarchically from networks of smaller ones in such a way that proofs of the whole are constructed from proofs of its components. We also show the consistency of our denotational models w.r.t. a simple operational semantics based on transitions which make internal process activity explicit.

Rapid Identification of Nine Microorganisms Causing Acute Respiratory Tract Infections by Single-Tube Multiplex Reverse Transcription-PCR: Feasibility Study

Abstract: Acute respiratory tract infections (ARIs) are leading causes of morbidity and, in developing countries, mortality in children. A multiplex reverse transcription-PCR (RT-PCR) assay was developed to allow in one test the detection of nine different microorganisms (enterovirus, influenza A and B viruses, respiratory syncytial virus [RSV], parainfluenzaviruses type 1 and type 3, adenovirus, Mycoplasma pneumoniae, and Chlamydia pneumoniae) that do not usually colonize the respiratory tracts of humans but, if present, must be assumed to be the cause of respiratory disease. Clinical samples from 1,118 children admitted to the Department of Pediatrics because of an ARI between November 1995 and April 1998 were used for a first clinical evaluation. Detection of one of the microorganisms included in the assay was achieved for 395 of 1,118 (35%) clinical samples, of which 37.5% were RSV, 20% were influenza A virus, 12.9% were adenovirus, 10.6% were enterovirus, 8.1% were M. pneumoniae, 4.3% were parainfluenzavirus type 3, 3.5% were parainfluenzavirus type 1, 2.8% were influenza B virus, and 0.2% were C. pneumoniae. Seasonal variations in the rates of detection of the different organisms were observed, as was expected from the literature. The levels of concordance with the data obtained by commercially available enzyme immunoassays were 95% for RSV and 98% for influenza A. The results show that the multiplex RT-PCR-enzyme-linked immunosorbent assay is a useful and rapid diagnostic tool for the management of children with ARI. Studies of the overall benefit of this method with regard to the use of antibiotics, the use of diagnostic procedures including additional microbiological tests, and hospitalization rate and duration are warranted.

PAF-mediated pulmonary edema: a new role for acid sphingomyelinase and ceramide

Abstract: Platelet-activating factor (PAF) induces pulmonary edema and has a key role in acute lung injury (ALI) Here we show that PAF induces pulmonary edema through two mechanisms: acid sphingomyelinase (ASM)-dependent production of ceramide, and activation of the cyclooxygenase pathway Agents that interfere with PAF-induced ceramide synthesis, such as steroids or the xanthogenate D609, attenuate pulmonary edema formation induced by PAF, endotoxin or acid instillation Our results identify acid sphingomyelinase and ceramide as possible therapeutic targets in acute lung injury