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Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules.

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TLDR
It is shown that proteins, enzymes and DNA rapidly induce the formation of protective metal-organic framework coatings under physiological conditions by concentrating the framework building blocks and facilitating crystallization around the biomacromolecules.
Abstract
Robust biomacromolecules could be used for a wide range of biotechnological applications. Here the authors report a biomimetic mineralization process, in which biomolecules are encapsulated within metal-organic frameworks, and their stability is subsequently increased without significant bioactivity loss.

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References
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Journal ArticleDOI

PQQ glucose dehydrogenase with novel electron transfer ability

TL;DR: The constructed fusion protein showed not only intra-molecular electron transfer, between PQQ and heme of the cytochrome c domain, but also electron transfer from heme to the electrode, thereby allowing the construction of a direct electron transfer-type glucose sensor.
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Reversible immobilization of urease onto Procion Brown MX-5BR-Ni(II) attached polyamide hollow-fibre membranes

TL;DR: In this paper, the maximum urease capacity of polyamide hollow-fibre membrane was 78 mg g −1 fibre and the retained adsorbed enzyme activity was found to be 37%.
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The Concept of Solvent Compatibility and Its Impact on Protein Stability and Activity Enhancement in Nonaqueous Solvents

TL;DR: In this article, Chen and Arnold (Proc. Natl. Acad. Sci. U.S.A. 1993, 90, 5618−5622) have generated a 10 amino acid mutant (PC3) of subtilisin E (SE) that has enhanced activity in mixed DMF/water solvent systems.
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New functional amorphous calcium phosphate nanocomposites by enzyme-assisted biomineralization.

TL;DR: The reported method paves the way for novel biomineralization via enzyme molecules to form functional enzymes containing nanocomposites using ACP-NCs, which retained about 43% of the catalytic activity of free carboxyl esterase.
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Biomineral-inspired growth of metal–organic frameworks in gelatin hydrogel matrices

TL;DR: A biomineral-inspired growth approach to the prototypical metal-organic frameworks (MOFs) ZIF-8 and HKUST-1 using cheap abundant non-toxic gelatin hydrogels as both a matrix and macromolecular additive for crystal growth.
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