Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
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The Past, Present, and Future of Genetic Manipulation in Toxoplasma gondii
TL;DR: The major strategies for T. gondii genetic manipulation including genetic crosses, insertional mutagenesis, chemical mutagenisation, homologous gene replacement, conditional knockdown techniques, and the recently developed clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system are summarized.
Journal ArticleDOI
On-chip multiplexed single-cell patterning and controllable intracellular delivery
Zaizai Dong,Yanli Jiao,Bingteng Xie,Yongcun Hao,Pan Wang,Yuanyuan Liu,Junfeng Shi,Chandani Chitrakar,Stephen Black,Yu-Chieh Wang,L. James Lee,Mo Li,Yubo Fan,Lingqian Chang,Lingqian Chang +14 more
TL;DR: A vacuum-assisted micropore array platform that enables massively parallel single-cell manipulation and the intracellular delivery of macromolecules and small molecules and provides a simple, efficient, high-throughput intrACEllular delivery method that may facilitate on-chip cell manipulation, intrace cellular investigation and cancer therapy.
Journal ArticleDOI
RNA-guided retargeting of Sleeping Beauty transposition in human cells
TL;DR: This work demonstrates biased genome-wide integration of the Sleeping Beauty (SB) transposon by combining it with components of the CRISPR/Cas9 system and provides proof-of-concept that it is possible to influence the target site selection of SB by fusing it to a catalytically inactive Cas9 and by providing a single guide RNA against the human Alu retrotransposon.
Posted ContentDOI
In vivo CRISPRa decreases seizures and rescues cognitive deficits in a rodent model of epilepsy
Gaia Colasante,Yichen Qiu,Luca Massimino,Claudia Di Berardino,Jonathan H Cornford,Albert Snowball,Mikail Weston,Steffan P Jones,Serena Giannelli,Andreas Lieb,Stephanie Schorge,Stephanie Schorge,Dimitri M. Kullmann,Vania Broccoli,Gabriele Lignani +14 more
TL;DR: In this article, the authors applied a doxycycline-inducible CRISPRa technology to increase the expression of the potassium channel gene Kcna1 (encoding Kv1.1) in mouse hippocampal excitatory neurons.
Journal ArticleDOI
Myoediting: Toward prevention of muscular dystrophy by therapeutic genome editing
TL;DR: A historical overview of genome-editing technologies is provided, the most recent advances are summarized, and potential strategies and challenges for permanently correcting genetic mutations that cause muscular dystrophies are discussed.
References
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Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Journal ArticleDOI
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
Bo Li,Colin N. Dewey +1 more
TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.
Journal ArticleDOI
Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.