Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
Citations
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Synthetic Biology Speeds Up Drug Target Discovery.
TL;DR: Synthetic biology works well in association with the CRISPR system for drug target functional screening and more and more well-designed circuits are developed to discover novel drug targets and precisely regulate drug therapy for diseases.
Journal ArticleDOI
Optimization of lentiviral transduction parameters and its application for CRISPR-based secretome modification of human endometrial mesenchymal stem cells.
Pavel Deryabin,Anastasiia Griukova,Alla N. Shatrova,Alexey Petukhov,Nikolay Nikolsky,Aleksandra Borodkina +5 more
TL;DR: The present study aimed to optimize hMESCs transduction parameters and apply Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 technology for genome and secretome modification and found that the use of Ps for hM ESCs genetic manipulations is preferable, as it has no impact on the stem-cell properties, whereas Pb application is undesirable.
Journal ArticleDOI
Characterization of Cas9–Guide RNA Orthologs
Jonathan L. Braff,Stephanie J. Yaung,Stephanie J. Yaung,Stephanie J. Yaung,Kevin M. Esvelt,George M. Church,George M. Church +6 more
TL;DR: The value of orthogonal Cas9-gRNA pairings that allow for concurrent activation and editing at independent target sites in the same cell are described and considerations for the desired specificity inCas9-coupled functions are discussed.
Journal ArticleDOI
CRISPR-Cpf1 Activation of Endogenous BMP4 Gene for Osteogenic Differentiation of Umbilical-Cord-Derived Mesenchymal Stem Cells
Jae-Hoon Choi,Taegeun Bae,Ninj Byambasuren,Seong-Ho Park,Chris Hyunchul Jo,Dokyoung Kim,Junho K. Hur,Nathaniel S. Hwang +7 more
TL;DR: It is observed that the CRISPR-dAsCpf1-VPR activator can be applied to enhance the osteogenic differentiation of human UC-MSCs, via increasing the expression level of endogenous BMP4 gene.
Journal ArticleDOI
CRISPR-engineered genome editing for the next generation neurological disease modeling.
TL;DR: Recent progress of CRISPR‐based brain disease modeling studies are reviewed and future requirement to tackle current difficulties in usage of these technologies are discussed.
References
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.