Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
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CRISPR-mediated activation of a promoter or enhancer rescues obesity caused by haploinsufficiency
Navneet Matharu,Rattanasopha S,Rattanasopha S,Serena Tamura,Lenka Maliskova,Yi Wang,Adelaide Bernard,Aaron Hardin,Walter L. Eckalbar,Christian Vaisse,Nadav Ahituv +10 more
TL;DR: By delivering this system into the mouse hypothalamus using adeno-associated virus, they rescued the obesity phenotype caused by haploinsufficiency of either of two genes known to promote obesity when mutated in mice and humans, highlighting the translational potential of the CRISPR activation system to treat haplo Insufficient disease.
Journal ArticleDOI
A CRISPR–Cas9-triggered strand displacement amplification method for ultrasensitive DNA detection
TL;DR: CRISDA is a powerful isothermal tool for ultrasensitive and specific detection of nucleic acids in point-of-care diagnostics and field analyses.
Journal ArticleDOI
A CRISPR-dCas Toolbox for Genetic Engineering and Synthetic Biology.
Xiaoshu Xu,Lei S. Qi +1 more
TL;DR: This review summarizes current applications of the dCas tools for transcription regulation, epigenetic engineering, genome imaging, genetic screens, and chromatin immunoprecipitation and highlights the advantages and existing challenges.
Journal ArticleDOI
Multiplexed barcoded CRISPR-Cas9 screening enabled by CombiGEM
Alan S.L. Wong,Gigi C G Choi,Cheryl H. Cui,Gabriela Pregernig,Pamela Milani,Miriam Adam,Samuel D. Perli,Samuel W. Kazer,Aleth Gaillard,Aleth Gaillard,Mario Hermann,Alex K. Shalek,Alex K. Shalek,Ernest Fraenkel,Timothy K. Lu +14 more
TL;DR: The CombiGEM-CRISPR platform will be applicable to a broad range of biological settings and will accelerate the systematic identification of genetic combinations and their translation into novel drug combinations that modulate complex human disease phenotypes.
Journal ArticleDOI
An Integrated Genome-wide CRISPRa Approach to Functionalize lncRNAs in Drug Resistance
Assaf C. Bester,Jonathan D. Lee,Jonathan D. Lee,Alejandro Chavez,Yu-Ru Lee,Yu-Ru Lee,Daphna Nachmani,Daphna Nachmani,Suhani Vora,Joshua Victor,Joshua Victor,Martin Sauvageau,Emanuele Monteleone,John L. Rinn,Paolo Provero,George M. Church,John G. Clohessy,Pier Paolo Pandolfi,Pier Paolo Pandolfi +18 more
TL;DR: A novel and powerful approach to identify integrated coding and non-coding pathways of therapeutic relevance to identify genetic units affecting sensitivity to cytarabine, the mainstay of treatment for acute myeloid leukemia (AML).
References
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The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.