Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
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Journal ArticleDOI
Interrogation of enhancer function by enhancer-targeting CRISPR epigenetic editing.
Kailong Li,Yuxuan Liu,Hui Cao,Yuannyu Zhang,Zhimin Gu,Xin Liu,Andy Yu,Pranita Kaphle,Kathryn E. Dickerson,Min Ni,Jian Xu +10 more
TL;DR: Improved CRISPR/dCas9-based enhancer-targeting epigenetic editing systems are described, showing more robust perturbations of enhancer activity and gene transcription with minimal off-targets.
Journal ArticleDOI
Assembly of CRISPR ribonucleoproteins with biotinylated oligonucleotides via an RNA aptamer for precise gene editing.
Jared Carlson-Stevermer,Amr A. Abdeen,Lucille Kohlenberg,Madelyn Goedland,Kaivalya Molugu,Meng Lou,Krishanu Saha +6 more
TL;DR: A modular RNA aptamer-streptavidin strategy, termed S1mplex, is developed to complex CRISPR-Cas9 ribonucleoproteins with a nucleic acid donor template, as well as other biotinylated molecules such as quantum dots, which could greatly reduce the time and cost of in vitro or ex vivo gene-editing applications in precision medicine and drug discovery.
Journal ArticleDOI
Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling.
Albert W. Cheng,Nathaniel Jillette,Phoebe Lee,Dylan Plaskon,Yasuhiro Fujiwara,Wenbo Wang,Aziz Taghbalout,Haoyi Wang +7 more
TL;DR: Cloning PUFa and PUFb were amplified from constructs containing these coding sequences with primers containing SgrAI and PacI sites and were used to replace Sgrai-dCas9-FseI from pAC164:pmax- dCas9Master_VP64 to create pAC1355:PMax-NLSPUFa-VP64 and pAC 1356:pmAX-NL SPUFb-VP 64.
Journal ArticleDOI
Beyond the RNA-dependent function of LncRNA genes.
TL;DR: Recent work on several lncRNAs genes demonstrates that not only is the produced RNA species important, but also that transcription of the lncRNA locus alone can have regulatory functions.
Journal ArticleDOI
dCas9-Based Scn1a Gene Activation Restores Inhibitory Interneuron Excitability and Attenuates Seizures in Dravet Syndrome Mice.
Gaia Colasante,Gabriele Lignani,Simone Brusco,Claudia Di Berardino,Jenna C Carpenter,Serena Giannelli,Nicholas Valassina,Simone Bido,Raffaele Ricci,Valerio Castoldi,Silvia Marenna,Timothy Church,Luca Massimino,Giuseppe Morabito,Fabio Benfenati,Fabio Benfenati,Stephanie Schorge,Letizia Leocani,Dimitri M. Kullmann,Vania Broccoli +19 more
TL;DR: The results pave the way for exploiting dCas9-based gene activation as an effective and targeted approach to DS and other disorders resulting from altered gene dosage.
References
More filters
Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Journal ArticleDOI
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
Bo Li,Colin N. Dewey +1 more
TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.
Journal ArticleDOI
Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.