Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
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Epigenetic Regulation of Interleukin 6 by Histone Acetylation in Macrophages and Its Role in Paraquat-Induced Pulmonary Fibrosis
Ling-Li Hu,Yanfang Yu,Huijie Huang,Han-Ting Fan,Li Hu,Caiyong Yin,Kai Li,David Fulton,Feng Chen,Feng Chen +9 more
TL;DR: It is demonstrated that PQ-induced IL-6 expression in macrophages plays a central role in pulmonary fibrosis through enhanced epithelial-to-mesenchymal transition (EMT) and was regulated dynamically by HDAC and HAT both in vitro and in vivo via epigenetically regulating chromatin accessibility.
Journal ArticleDOI
Multiplexed CRISPR Activation of Cryptic Sugar Metabolism Enables Yarrowia Lipolytica Growth on Cellobiose.
TL;DR: This work expands the synthetic biology toolbox for metabolic engineering in Y. lipolytica and demonstrates how the programmability of the CRISPR-Cas9 system can enable facile investigation of transcriptionally silent regions of the genome.
Journal ArticleDOI
In vivo CRISPRa decreases seizures and rescues cognitive deficits in a rodent model of epilepsy.
Gaia Colasante,Yichen Qiu,Luca Massimino,Claudia Di Berardino,Jonathan H Cornford,Albert Snowball,Mikail Weston,Steffan P Jones,Serena Giannelli,Andreas Lieb,Stephanie Schorge,Stephanie Schorge,Dimitri M. Kullmann,Vania Broccoli,Gabriele Lignani +14 more
TL;DR: It is reported thatCRISPRa upregulation of an endogenous potassium channel reduces seizures and rescues cognitive defects in an epilepsy model, providing proof of principle for a translational CRISPR-based approach to treat neurological diseases.
Journal ArticleDOI
Integrated design, execution, and analysis of arrayed and pooled CRISPR genome-editing experiments
Matthew C. Canver,Maximilian Haeussler,Daniel E. Bauer,Stuart H. Orkin,Neville E. Sanjana,Ophir Shalem,Guo-Cheng Yuan,Feng Zhang,Jean-Paul Concordet,Luca Pinello,Luca Pinello +10 more
TL;DR: This protocol allows for design and execution of arrayed and pooled CRISPR experiments in 4-5 weeks by non-experts, as well as computational data analysis that can be performed in 1-2 d by both computational and noncomputational biologists alike using web-based and/or command-line versions.
Journal ArticleDOI
Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Lin Lin,Yong Liu,Fengping Xu,Jinrong Huang,Tina Fuglsang Daugaard,Trine Skov Petersen,Bettina Hansen,Lingfei Ye,Qing Zhou,Fang Fang,Ling Yang,Shengting Li,Lasse Fløe,Kristopher Torp Jensen,Ellen Shrock,Fang Chen,Huanming Yang,Jian Wang,Xin Liu,Xun Xu,Lars Bolund,Anders Lade Nielsen,Yonglun Luo +22 more
TL;DR: It is proved that dCas9 methyltransferases cause efficient RNA-guided methylation of specific endogenous CpGs and can mediate transient inhibition of gene expression, which might be caused by d Cas9-mediated de novo DNA methylation as well as interference with transcription.
References
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Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.