Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
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Loss of XIST in Breast Cancer Activates MSN-c-Met and Reprograms Microglia via Exosomal miRNA to Promote Brain Metastasis
Fei Xing,Yin Liu,Shih-Ying Wu,Kerui Wu,Sambad Sharma,Yin-Yuan Mo,Jiamei Feng,Stephanie Sanders,Guangxu Jin,Ravi Singh,Pierre-Alexandre Vidi,Abhishek Tyagi,Michael D. Chan,Jimmy Ruiz,Waldemar Debinski,Boris Pasche,Hui-Wen Lo,Linda J. Metheny-Barlow,Ralph B. D'Agostino,Kounosuke Watabe +19 more
TL;DR: Mechanisms of how loss of the lncRNA XIST promotes brain metastasis in breast cancer are described and fludarabine is identified as a potential therapeutic agent that specifically eliminates XISTlow tumor cells in the brain.
Journal ArticleDOI
Towards CRISPR/Cas crops - bringing together genomics and genome editing.
TL;DR: It is argued that overcoming technical and social barriers to the application of genome editing will allow this technology to produce a new generation of high-yielding, climate ready crops.
Journal ArticleDOI
Massively parallel Cas13 screens reveal principles for guide RNA design
Hans-Hermann Wessels,Alejandro Méndez-Mancilla,Xinyi Guo,Mateusz Legut,Zharko Daniloski,Neville E. Sanjana +5 more
TL;DR: It is shown that Cas13 can be used in forward transcriptomic pooled screens and predicted optimized Cas13 gRNAs for all protein-coding transcripts in the human genome by computational modeling of large-scale screening data.
Journal ArticleDOI
Continuous evolution of SpCas9 variants compatible with non-G PAMs.
Shannon M. Miller,Tina Wang,Tina Wang,Tina Wang,Peyton B. Randolph,Peyton B. Randolph,Peyton B. Randolph,Mandana Arbab,Mandana Arbab,Mandana Arbab,Max W. Shen,Tony P. Huang,Tony P. Huang,Tony P. Huang,Zaneta Matuszek,Gregory A. Newby,Gregory A. Newby,Gregory A. Newby,Holly A. Rees,Holly A. Rees,Holly A. Rees,David R. Liu,David R. Liu,David R. Liu +23 more
TL;DR: Three new SpCas9 variants that collectively recognize NRNH PAMs are reported that enable targeting of most NR PAM sequences and substantially reduce the fraction of genomic sites that are inaccessible by Cas9-based methods.
Journal ArticleDOI
Writing of H3K4Me3 overcomes epigenetic silencing in a sustained but context-dependent manner
David Cano-Rodriguez,Rutger A. F. Gjaltema,Laura J Jilderda,Pytrick Jellema,Jelleke Dokter-Fokkens,Marcel H. J. Ruiters,Marianne G. Rots +6 more
TL;DR: This work demonstrates sustained gene re-expression using two types of engineered DNA-binding domains fused to a H3K4 methyltransferase to uncover potent epigenetic modifications to be directly written onto genomic loci to stably activate any given gene.
References
More filters
Journal ArticleDOI
Fast gapped-read alignment with Bowtie 2
TL;DR: Bowtie 2 combines the strengths of the full-text minute index with the flexibility and speed of hardware-accelerated dynamic programming algorithms to achieve a combination of high speed, sensitivity and accuracy.
Journal ArticleDOI
RSEM: accurate transcript quantification from RNA-Seq data with or without a reference genome
Bo Li,Colin N. Dewey +1 more
TL;DR: It is shown that accurate gene-level abundance estimates are best obtained with large numbers of short single-end reads, and estimates of the relative frequencies of isoforms within single genes may be improved through the use of paired- end reads, depending on the number of possible splice forms for each gene.
Journal ArticleDOI
A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity.
Martin Jinek,Krzysztof Chylinski,Krzysztof Chylinski,Ines Fonfara,Michael H. Hauer,Jennifer A. Doudna,Emmanuelle Charpentier +6 more
TL;DR: This study reveals a family of endonucleases that use dual-RNAs for site-specific DNA cleavage and highlights the potential to exploit the system for RNA-programmable genome editing.
Journal ArticleDOI
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity
Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
TL;DR: The results indicate that large, annotated cell-line collections may help to enable preclinical stratification schemata for anticancer agents and the generation of genetic predictions of drug response in the preclinical setting and their incorporation into cancer clinical trial design could speed the emergence of ‘personalized’ therapeutic regimens.
Journal ArticleDOI
Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.