Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
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Journal ArticleDOI
Precision Control of CRISPR-Cas9 Using Small Molecules and Light
Soumyashree A. Gangopadhyay,Soumyashree A. Gangopadhyay,Soumyashree A. Gangopadhyay,Kurt J. Cox,Debasish Manna,Debasish Manna,Debasish Manna,Donghyun Lim,Basudeb Maji,Basudeb Maji,Basudeb Maji,Qingxuan Zhou,Amit Choudhary,Amit Choudhary,Amit Choudhary +14 more
TL;DR: A perspective on advances in the precision control of Cas9 over aforementioned dimensions using external stimuli (e.g., small molecules or light) for controlled activation, inhibition, or degradation of Cas 9 is provided.
Journal ArticleDOI
A Neuron-Optimized CRISPR/dCas9 Activation System for Robust and Specific Gene Regulation.
Katherine E. Savell,Svitlana V. Bach,Morgan E. Zipperly,Jasmin S. Revanna,Nicholas A. Goska,Jennifer J. Tuscher,Corey G. Duke,Faraz A. Sultan,Julia N. Burke,Derek M. Williams,Lara Ianov,Jeremy J. Day +11 more
TL;DR: A highly efficient, neuron-optimized dual lentiviral CRISPR-based transcriptional activation (CRISPRa) system capable of robust, modular, and tunable gene induction and multiplexed gene regulation across several primary rodent neuron culture systems is demonstrated.
Journal ArticleDOI
LncRNA AK023948 is a positive regulator of AKT.
Pratirodh Koirala,Jianguo Huang,Tsui-Ting Ho,Fangting Wu,Xianfeng Ding,Xianfeng Ding,Yin-Yuan Mo +6 more
TL;DR: This study demonstrates two previously uncharacterized factors AK023948 and DHX9 as important players in the AKT pathway, and that their upregulation may contribute to breast tumour progression.
Journal ArticleDOI
Heterochromatin-Encoded Satellite RNAs Induce Breast Cancer
Quan Zhu,Nien Hoong,Aaron Aslanian,Aaron Aslanian,Toshiro Hara,Christopher Benner,Sven Heinz,Karen H. Miga,Eugene Ke,Sachin Verma,Jan Soroczynski,John R. Yates,Tony Hunter,Inder M. Verma +13 more
TL;DR: Evidence is provided that increased levels of satellite RNAs in mammary glands induce tumor formation in mice, and it is shown that genomic instability induced by satelliteRNAs occurs through interactions with BRCA1-associated protein networks required for the stabilization of DNA replication forks.
Journal ArticleDOI
An RNA-aptamer-based two-color CRISPR labeling system.
Siyuan Wang,Siyuan Wang,Jun-Han Su,Jun-Han Su,Feng Zhang,Feng Zhang,Feng Zhang,Xiaowei Zhuang,Xiaowei Zhuang +8 more
TL;DR: This work reports the development of an alternative two-color CRISPR labeling method using only the well-characterized Streptococcus pyogenes Cas9, by incorporating MS2 or PP7 RNA aptamers into the sgRNA.
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.