Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
About:
The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
Citations
More filters
Journal ArticleDOI
Unique features of long non-coding RNA biogenesis and function
Jeffrey J. Quinn,Howard Y. Chang +1 more
TL;DR: This Review describes special events in the lifetimes of lncRNAs — before, during and after transcription — and discusses how these events ultimately shape the unique characteristics and functional roles of lNCRNAs.
Journal ArticleDOI
The emerging role of lncRNAs in cancer
TL;DR: The strategies that led to the identification of cancer-related lncRNAs and the methodologies and challenges involving the study of these molecules are discussed, as well as the imminent applications of these findings to the clinic.
Journal ArticleDOI
Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers
Isaac B. Hilton,Anthony D'Ippolito,Christopher M. Vockley,Pratiksha I. Thakore,Gregory E. Crawford,Timothy E. Reddy,Charles A. Gersbach +6 more
TL;DR: A programmable, CRISPR-Cas9-based acetyltransferase consisting of the nuclease-null dCas9 protein fused to the catalytic core of the human acetyl transferase p300 is described, leading to robust transcriptional activation of target genes from promoters and both proximal and distal enhancers.
Crystal Structure of Cas9 in Complex with Guide RNA and Target DNA
Hiroshi Nishimasu,Naoshi Dohmae,Ryuichiro Ishitani,Osamu Nureki,Fei Ran,Patrick D. Hsu,Silvana Konermann,Soraya I. Shehata,Feng Zhang +8 more
TL;DR: The crystal structure of Streptococcus pyogenes Cas9 in complex with sgRNA and its target DNA at 2.5 Å resolution and accompanying functional analyses have revealed the molecular mechanism of RNA-guided DNA targeting by Cas9, paving the way for the rational design of new, versatile genome-editing technologies.
Journal ArticleDOI
Highly efficient Cas9-mediated transcriptional programming
Alejandro Chavez,Jonathan Scheiman,Suhani Vora,Benjamin W. Pruitt,Marcelle Tuttle,Eswar Prasad Ramachandran Iyer,Shuailiang Lin,Samira Kiani,Christopher D. Guzman,Daniel J. Wiegand,Dmitry Ter-Ovanesyan,Jonathan L. Braff,Noah Davidsohn,Benjamin E. Housden,Norbert Perrimon,Ron Weiss,John Aach,James J. Collins,George M. Church +18 more
TL;DR: An improved transcriptional regulator obtained through the rational design of a tripartite activator, VP64-p65-Rta (VPR), fused to nuclease-null Cas9 is described and demonstrated in activating endogenous coding and noncoding genes and stimulating neuronal differentiation of human induced pluripotent stem cells (iPSCs).
References
More filters
Journal ArticleDOI
Modeling genomic diversity and tumor dependency in malignant melanoma
William M. Lin,Alissa C. Baker,Rameen Beroukhim,Wendy Winckler,Whei Feng,Jennifer M. Marmion,Elisabeth Laine,Heidi Greulich,Hsiuyi Tseng,Casey Gates,F. Stephen Hodi,Glenn Dranoff,William R. Sellers,Roman K. Thomas,Matthew Meyerson,Todd R. Golub,Reinhard Dummer,Meenhard Herlyn,Gad Getz,Levi A. Garraway +19 more
TL;DR: It is shown that cultured melanoma cells encompass the spectrum of significant genomic alterations present in primary tumors, and genetically defined cell culture collections offer a rich framework for systematic functional studies in melanoma and other tumors.
Journal ArticleDOI
Registered Report: COT drives resistance to RAF inhibition through MAP kinase pathway reactivation
TL;DR: This Registered Report describes the proposed replication plan of key experiments from “COT drives resistance to RAF inhibition through MAPK pathway reactivation” by Johannessen and colleagues, published in Nature in 2010, and the results of the replications will be published in eLife.
Journal ArticleDOI
Activation Domain–Mediated Targeting of the SWI/SNF Complex to Promoters Stimulates Transcription from Nucleosome Arrays
Kristen E. Neely,Ahmed H. Hassan,Annika E. Wallberg,David J. Steger,Bradley R. Cairns,Anthony P. H. Wright,Jerry L. Workman +6 more
TL;DR: It is shown that the SWI/SNF complex stimulated in vitro transcription from nucleosome templates in an activation domain-dependent manner.
Journal ArticleDOI
Combinatorial Treatments That Overcome PDGFRβ-Driven Resistance of Melanoma Cells to V600EB-RAF Inhibition
TL;DR: A targeted combinatorial strategy to overcome PLX4032/vemurafenib resistance in melanoma cell lines or short-term culture where the resistance is driven by PDGFRβ upregulation, achieving synergistic growth inhibition and cytotoxic response is defined.
Journal ArticleDOI
A novel mediator of class II gene transcription with homology to viral immediate-early transcriptional regulators
Marcus Kretzschmar,Marcus Kretzschmar,Klaus Kaiser,Friedrich Lottspeich,Michael Meisterernst +4 more
TL;DR: Structural and functional similarities, including sequence homology to domains essential for cofactor function, cofactor activity, promiscuity with respect to transcriptional activators, and interactions with components of the basal transcription machinery, relate this novel cellular cofactor to viral immediate-early transcriptional regulators.