Open Access
Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
Silvana Konermann,Mark D. Brigham,Alexandro E. Trevino,Julia Joung,Clea Barcena,Patrick D. Hsu,Naomi Habib,Jonathan S. Gootenberg,Hiroshi Nishimasu,Osamu Nureki,Feng Zhang,Omar O. Abudayyeh +11 more
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The article was published on 2016-05-22 and is currently open access. It has received 1792 citations till now. The article focuses on the topics: CRISPR.read more
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Journal ArticleDOI
CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes.
Alexis C. Komor,Alexis C. Komor,Alexis C. Komor,Ahmed H. Badran,Ahmed H. Badran,Ahmed H. Badran,David R. Liu,David R. Liu,David R. Liu +8 more
TL;DR: Recent developments that extend the generality, DNA specificity, product selectivity, and fundamental capabilities of natural CRISPR systems are described.
Journal ArticleDOI
RNA N6-methyladenosine methyltransferase-like 3 promotes liver cancer progression through YTHDF2-dependent posttranscriptional silencing of SOCS2
Mengnuo Chen,Lai Wei,Cheuk-Ting Law,Felice Ho-Ching Tsang,Jialing Shen,Carol Lai-Hung Cheng,Long-Hin Tsang,Daniel W.H. Ho,David Kung-Chun Chiu,Joyce Man-Fong Lee,Carmen Chak-Lui Wong,Irene Oi-Lin Ng,Chun-Ming Wong +12 more
TL;DR: Methyltransferase‐like 3 (METTL3), a major RNA N6‐adenosine methyltransferase, was significantly up‐regulated in human hepatocellular carcinoma (HCC) and multiple solid tumors and suggest an important mechanism of epigenetic alteration in liver carcinogenesis.
Journal ArticleDOI
The next generation of CRISPR-Cas technologies and applications.
TL;DR: The CRISPR–Cas toolkit has been expanding to include single-base editing enzymes, targeting RNA and fusing inactive Cas proteins to effectors that regulate various nuclear processes, and the new advances are considerably improving the authors' understanding of biological processes and are propelling CRISpr–Cas-based tools towards clinical use in gene and cell therapies.
Journal ArticleDOI
Off-target Effects in CRISPR/Cas9-mediated Genome Engineering
TL;DR: The improvement off-target specificity in the CRISPR/Cas9 system will provide solid genotype–phenotype correlations, and thus enable faithful interpretation of genome-editing data, which will certainly facilitate the basic and clinical application of this technology.
Journal ArticleDOI
A revised airway epithelial hierarchy includes CFTR-expressing ionocytes
Daniel T. Montoro,Adam L. Haber,Moshe Biton,Moshe Biton,Vladimir Vinarsky,Brian M. Lin,Susan E. Birket,Feng Yuan,Sijia Chen,Hui Min Leung,Jorge Villoria,Noga Rogel,Grace Burgin,Alexander M. Tsankov,Avinash Waghray,Michal Slyper,Julia Waldman,Lan Nguyen,Danielle Dionne,Orit Rozenblatt-Rosen,Purushothama Rao Tata,Hongmei Mou,Manjunatha Shivaraju,Hermann Bihler,Martin Mense,Guillermo J. Tearney,Steven M. Rowe,John F. Engelhardt,Aviv Regev,Aviv Regev,Jayaraj Rajagopal +30 more
TL;DR: ‘pulse-seq’ is developed, combining scRNA-seq and lineage tracing, to show that tuft, neuroendocrine and ionocyte cells are continually and directly replenished by basal progenitor cells, establishing a new cellular narrative for airways disease.
References
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Jordi Barretina,Giordano Caponigro,Nicolas Stransky,Kavitha Venkatesan,Adam A. Margolin,Adam A. Margolin,Sungjoon Kim,Christine D. Wilson,Joseph Lehar,Gregory V. Kryukov,Dmitriy Sonkin,Anupama Reddy,Manway Liu,Lauren Murray,Michael F. Berger,Michael F. Berger,John Monahan,Paula Morais,Jodi Meltzer,Adam Korejwa,Judit Jané-Valbuena,Judit Jané-Valbuena,Felipa A. Mapa,Joseph Thibault,Eva Bric-Furlong,Pichai Raman,Aaron Shipway,Ingo H. Engels,Jill Cheng,Guoying K. Yu,Jianjun Yu,Peter Aspesi,Melanie de Silva,Kalpana Jagtap,Michael D. Jones,Li Wang,Charlie Hatton,Emanuele Palescandolo,Supriya Gupta,Scott Mahan,Carrie Sougnez,Robert C. Onofrio,Ted Liefeld,Laura E. MacConaill,Wendy Winckler,Michael R. Reich,Nanxin Li,Jill P. Mesirov,Stacey Gabriel,Gad Getz,Kristin G. Ardlie,Vivien W. Chan,Vic E. Myer,Barbara L. Weber,Jeffrey A. Porter,Markus Warmuth,Peter Finan,Jennifer L. Harris,Matthew Meyerson,Matthew Meyerson,Todd R. Golub,Michael Morrissey,William R. Sellers,Robert Schlegel,Levi A. Garraway,Levi A. Garraway +65 more
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Molecular signatures database (MSigDB) 3.0
Arthur Liberzon,Aravind Subramanian,Reid M. Pinchback,Helga Thorvaldsdottir,Pablo Tamayo,Jill P. Mesirov +5 more
TL;DR: A new version of the database, MSigDB 3.0, is reported, with over 6700 gene sets, a complete revision of the collection of canonical pathways and experimental signatures from publications, enhanced annotations and upgrades to the web site.